¸¸¼º À°¾ÆÁ¾¼º ¿°ÁõÀÇ ÆĶóÇÉÆ÷¸Å Á¶Á÷¿¡¼ ´Ù¾çÇÑ Ç×»ê±Õ °ËÃâ¹ýÀÇ ºñ±³
Comparison of Various Detection Methods of Mycobacterium Species in Formalin-Fixed Paraffin-Embedded Tissue with Chronic Granulomatous Inflammation
ÀÌÇö½Â, ÀÌÇü³², ÀÓ¼Ò¿µ, ÀÌ¿¬¼ö, À̱³¿µ, ÃÖ¿µÁø,
¼Ò¼Ó »ó¼¼Á¤º¸
ÀÌÇö½Â ( Lee Hyun-Seung )
°¡Å縯´ëÇб³ ÀÇ°ú´ëÇÐ º´¿øº´¸®Çб³½Ç
ÀÌÇü³² ( Lee Hyoung-Nam )
°¡Å縯´ëÇб³ ÀÇ°ú´ëÇÐ º´¿øº´¸®Çб³½Ç
ÀÓ¼Ò¿µ ( Im So-Young )
°¡Å縯´ëÇб³ ÀÇ°ú´ëÇÐ º´¿øº´¸®Çб³½Ç
ÀÌ¿¬¼ö ( Lee Youn-Soo )
°¡Å縯´ëÇб³ ÀÇ°ú´ëÇÐ º´¿øº´¸®Çб³½Ç
À̱³¿µ ( Lee Kyo-Young )
°¡Å縯´ëÇб³ ÀÇ°ú´ëÇÐ º´¿øº´¸®Çб³½Ç
ÃÖ¿µÁø ( Choi Yeong-Jin )
°¡Å縯´ëÇб³ ÀÇ°ú´ëÇÐ º´¿øº´¸®Çб³½Ç
KMID : 0357920100440030259
Abstract
Background: To determine the most effective method for detecting mycobacteria in formalin- fixed paraffin-embedded (FFPE) tissue, we compared the results of Ziehl-Neelsen stain (ZNS) and mycobacterial culture with those of polymerase chain reaction (PCR) and real-time quantitative PCR (RQ-PCR).
Methods: We analyzed 54 cases diagnosed as chronic granulomatous inflammation. In all cases, ZNS and nested PCR using three different primers, IS6110, Mpb64 and IS6110/Rpo¥â were done. RQ-PCR with the IS6110/Rpo¥â primer was done in 51 cases.
Results: Mycobacteria were identified by ZNS in 15/54 (27.8%) cases. RQ-PCR had the highest sensitivity (80.0%) compared to PCR with IS6110 (73.3%), Mpb64 (60.0%) and IS6110/Rpo¥â (73.3%). Specificity was higher in all PCR experiments (79.5-82.1%) than in RQ-PCR (69.4%) experiments. The false negative rate was lowest for RQ-PCR (20.0%) than for PCR with IS6110 (26.7%), Mpb64 (40.0%) and IS6110/Rpo¥â (26.7%). The false positive rate was highest for RQ-PCR (30.6%) compared to PCR with IS6110 (20.5%), Mpb64 (17.9%) and IS6110/Rpo¥â (20.5%).
Conclusions: RQ-PCR had the highest sensitivity, and the lowest false negative rate, but it also had a higher false positive rate than PCR for detection of mycobacteria in FFPE tissues.
Å°¿öµå
Polymerase chain reaction;Stain;Mycobacterium;Granulomatous disease;chronic
¿ø¹® ¹× ¸µÅ©¾Æ¿ô Á¤º¸
µîÀçÀú³Î Á¤º¸