Gene Expression Profiles of Uterine Normal Myometrium and Leiomyoma and Their Estrogen Responsiveness In Vitro
이은주, 프라티 바즈라촤리여, 이동목, 조경현, 김극준, 배영경, 김미진, 이기호, 김항진, 송건호, 최인호,
소속 상세정보
이은주 ( Lee Eun-Ju )
영남대학교 생명공학과
프라티 바즈라촤리여 ( Prati Bajracharya )
영남대학교 생명공학과
이동목 ( Lee Dong-Mok )
영남대학교 생명공학과
조경현 ( Cho Kyung-Hyun )
영남대학교 생명공학부
김극준 ( Kim Keuk-Jun )
영남대학교 의과대학 병리학교실
배영경 ( Bae Young-Kyung )
영남대학교 의과대학 병리학교실
김미진 ( Kim Mi-Jin )
영남대학교 의과대학 병리학교실
이기호 ( Lee Ki-Ho )
을지대학교 의과대학 생화학 분자생물학
김항진 ( Kim Hang-Jin )
경북대학교 의과대학 산부인과학교실
송건호 ( Song Gun-Ho )
경북대학교 의과대학 산부인과학교실
최인호 ( Choi In-Ho )
영남대학교 생명공학과
KMID : 0357920100440030272
Abstract
Background: Uterine leiomyomas are common benign smooth muscle tumors among the reproductive aged-women. The research has been aimed to identify the differentially expressed genes between normal myometrium and leiomyoma and to investigate the effects of E2 on their expression.
Methods: Gene microarray analysis was performed to identify the differentially expressed genes between normal myomerium and leiomyoma. The data was confirmed at protein level by tissue microarray.
Results: Gene microarray analysis revealed 792 upregulated genes in leiomyoma. Four genes (tropomyosin 4 [TPM4], collagen, type IV, alpha 2 [COL4α2], insulin-like growth factor binding protein 5 [IGFBP5], tripartite motif-containing 28 [TRIM28]) showed the most dramatic upregulation in all leiomyoma samples. Tissue microarray analyses of 262 sample pairs showed significantly elevated expression of TPM4, IGFBP5, estrogen receptor-α, and progesterone receptor (PR) protein in leiomyoma from the patients in their forties, COL4α2 in the forties and fifties age-groups, and TRIM28 in the thirties age-group. PR, insulin-like growth factor 1 (IGF-1), IGF-1 receptor (IGF-1R) and IGFBP5 were induced by E2 in in vitro culture of tissue explants from which cells migrated throughout the plate. Among these, PR, IGF-1, IGFBP5 genes showed higher expression in tissue compared to cells-derived from tissue in leiomyoma and IGF-1R in leiomyoma cell.
Conclusions: This observation implies the importance of the whole tissue context including the cells-derived from tissue in the research for the understanding of molecular mechanism of leiomyoma. Here, we report higher expression of TRIM28 in leiomyoma for the first time and identify E2-responsive genes that may have important roles in leiomyoma development.
키워드
Leiomyoma;17beta-estradiol;Uterus;Oligonucleotide array sequence analysis;Immunohistochemistry
원문 및 링크아웃 정보
등재저널 정보