에스트로겐 수용체-알파 녹아웃 마우스에 발생한 난소 출혈다낭의 병리학적 특징과 출혈다낭에서의 에스트로겐 수용체-알파의 역할
Pathologic Characteristics of Ovarian Hemorrhagic Polycyst in Estrogen Receptor-alpha (ERα) Knockout Mice and Roles of ERα in Hemorrhagic Polycyst
손현진, 김주헌, 이혜경, 박미자, 강동욱, 고제명,
소속 상세정보
손현진 ( Son Hyun-Jin )
을지대학교 의과대학 병리학교실
김주헌 ( Kim Ju-Hun )
을지대학교 의과대학 병리학교실
이혜경 ( Lee Hye-Kyung )
을지대학교 의과대학 병리학교실
박미자 ( Park Mi-Ja )
을지대학교 의과대학 병리학교실
강동욱 ( Kang Dong-Wook )
을지대학교 의과대학 병리학교실
고제명 ( Ko Che-Myong )
켄터키대학교 생식과학부 임상과학교실
KMID : 0357920100440040376
Abstract
Background: Polycystic ovary syndrome (PCOS) is the most common endocrinopathy causing anovulation in women of childbearing age. It has been well established that estrogen receptor-alpha knockout (ERαKO) mice display several pathologic ovarian phenotypes of PCOS. The aims of this study were to determine ovarian pathology in new ERαKO mice using a CreloxP approach and intra-ovarian ERα function as regulating key aspects of PCOS.
Methods: ERαKO mice, which were deficient in exon 3 of the ERα gene, were used. Immunohistochemical studies were done on ovaries of control and ERαKO mice using antibodies specific to ERα, ERβ, inhibin-α, and α-smooth muscle actin (SMA), as well as histochemical staining using Sudan black-B.
Results: All ovaries of ERαKO mice were larger than control mouse ovaries and displayed a disrupted theca-interstitial tissue organization, multiple atretic follicles and multiple hemorrhagic cysts. None of the ERαKO mouse ovaries showed a corpus luteum. In addition, heavy deposition of Sudan black-B positive foamy cells was seen. The theca externa of preantral immature follicles and hemorrhagic cysts showed strong expression of α-SMA.
Conclusions: ERαKO mice show hemorrhagic polycystic ovaries and hyperplasia of the theca externa. This study demonstrates that the ERα is the functional key to the pathogenesis of PCOS.
키워드
Polycystic ovary syndrome; Estrogen receptor-alpha knockout (ERαKO); Hemorrhagic polycysts; Immunohistochemistry
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