Relationship between the Endogenous Hypoxic Markers Hypoxia Inducible Factor-1α , Carbonic Anhydrase IX, and Epithelial Mesenchymal Transition Regulator TWIST Expression in Non-small Cell Lung Cancer
이정희, 박원영, 정성묵, 이민기, 김영대, 신동훈, 이창훈,
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이정희 ( Lee Jung-Hee )
부산대학교 의과대학 병리학교실
박원영 ( Park Won-Young )
부산대학교 의과대학 병리학교실
정성묵 ( Jeong Seong-Muk )
부산대학교 의과대학 병리학교실
이민기 ( Lee Min-Ki )
부산대학교 의과대학 내과학교실
김영대 ( Kim Young-Dae )
부산대학교 의학전문대학원 흉부외과학교실
신동훈 ( Shin Dong-Hoon )
부산대학교 의과대학 병리학교실
이창훈 ( Lee Chang-Hun )
부산대학교 의과대학 병리학교실
KMID : 0357920100440050469
Abstract
Background : The epithelial mesenchymal transition (EMT) is intimately associated with tumor hypoxia. The present study was conducted to investigate the immunohistochemical relationship between hypoxic and EMT-related molecules in non-small cell lung carcinoma (NSCLC).
Methods : Immunohistochemical staining for hypoxia inducible factor (HIF)-1a, carbonic anhydrase (CA) IX, TWIST, and E-cadherin proteins was performed in 146 cases of NSCLC (80 cases of adenocarcinoma and 66 cases of squamous cell carcinoma) using tissue microarray blocks.
Results : HIF-1a, TWIST, CA IX, and E-cadherin were expressed in 58 (40%), 90 (62%), 82 (56%), and 36 (25%) of 146 NSCLC cases, respectively. TWIST expression was positively correlated with HIF-1a expression (p = 0.03) and inversely correlated with E-cadherin expression (p < 0.01). TWIST and CA IX expression were not significantly interrelated, but each showed a relationship with histological tumor grade. However, the expression of these molecules had no significant effect on clinical staging or patient survival.
Conclusions : Although TWIST expression was correlated positively with HIF-1a expression and inversely correlated with E-cadherin, HIF-1a expression was not associated with E-cadherin expression. However, considering the relationship between HIF-1a and TWIST expression, further studies should be performed to demonstrate the role of hypoxia-induced EMT in NSCLC.
키워드
Carcinoma; non-small-cell lung; Epithelial mesenchymal transition; Immunohistochemistry
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