Evaluation of the HPV ISH Assay in Cervical Cancer
이정의, 신정하, 김종옥, 최영진, 이교영, 박종섭, 이원철, 이아원,
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이정의 ( Lee Jung-Uee )
가톨릭대학교 의과대학 병원병리학교실
신정하 ( Shin Jung-Ha )
가톨릭대학교 의과대학 병원병리학교실
김종옥 ( Kim Jong-Ok )
가톨릭대학교 의과대학 병리학교실
최영진 ( Choi Yeong-Jin )
가톨릭대학교 의과대학 병원병리학교실
이교영 ( Lee Kyo-Young )
가톨릭대학교 의과대학 병원병리학교실
박종섭 ( Park Jong-Sup )
가톨릭대학교 의과대학 산부인과학교실
이원철 ( Lee Won-Chul )
가톨릭대학교 의과대학 예방의학교실
이아원 ( Lee Ah-Won )
가톨릭대학교 의과대학 병원병리학교실
KMID : 0357920100440050513
Abstract
Background : Human papillomavirus (HPV) infection can be detected by in situ hybridization (ISH), in which a punctate signal pattern indicates integrated HPV DNA and a diffuse pattern denotes the presence of episomal viral DNA. This study was conducted to evaluate the usefulness of an HPV ISH assay for invasive cervical cancer.
Methods : The HPV ISH assay for high-risk HPV and immunohistochemical staining for p16INK4a, p53, bcl-2, and Ki-67 were performed in a tissue microarray of 279 cervical cancers.
Results : High-risk HPV ISH was positive in 194 (69.5%) of the samples. Punctate, diffuse, and mixed signal patterns were observed in 157 (56.3%), one (0.4%), and 36 cases (12.9%), respectively. Positive results in high-risk HPV ISH were associated with p16 and bcl-2 expression (p = 0.01 and p < 0.01, respectively). According to a Cox regression analysis, HPV infection and its surrogate immunohistochemical markers such as p16, bcl-2, and Ki-67 were not independent prognostic factors, but stage and grade were independent prognostic factors.
Conclusions : Our results confirm that an HPV ISH assay is reasonably sensitive for HPV infection and that it might be useful to identify integrated HPV DNA in formalin-fixed and paraffin-embedded specimens. Further study encompassing HPV type, E2/E6 ratio, and therapeutic modality is necessary to understand the clinical meaning of HPV status in cervical cancer.
키워드
HPV; In situ hybridization; Cervical cancer
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