Copy Number Alterations of BCAS1 in Squamous Cell Carcinomas
김유임, 이아원, Kim Jennifer, 이범희, 이성학, 남석우, 이석형, 박원상, 유남진, 이정용, 김상호, 김수영,
소속 상세정보
김유임 ( Kim Yu-Im )
Catholic University College of Medicine Department of Pathology
이아원 ( Lee Ah-Won )
Catholic University College of Medicine Department of Hospital Pathology
( Kim Jennifer )
Catholic University College of Medicine Department of Pathology
이범희 ( Lee Bum-Hee )
Catholic University College of Medicine Department of Pathology
이성학 ( Lee Sung-Hak )
Catholic University College of Medicine Department of Pathology
남석우 ( Nam Suk-Woo )
Catholic University College of Medicine Department of Pathology
이석형 ( Lee Sug-Hyung )
Catholic University College of Medicine Department of Pathology
박원상 ( Park Won-Sang )
Catholic University College of Medicine Department of Pathology
유남진 ( Yoo Nam-Jin )
Catholic University College of Medicine Department of Pathology
이정용 ( Lee Jung-Young )
Catholic University College of Medicine Department of Pathology
김상호 ( Kim Sang-Ho )
Catholic University College of Medicine Department of Pathology
김수영 ( Kim Su-Young )
Catholic University College of Medicine Department of Pathology
KMID : 0357920110450030271
Abstract
Background : Breast carcinoma amplified sequence 1 (BCAS1), located in 20q13, is amplified and overexpressed in breast cancers. Even though BCAS1 is expected to be an oncogene candidate, its contribution to tumorigenesis and copy number status in other malignancies is not reported. To elucidate the role of BCAS1 in squamous cell carcinomas, we investigated the copy number status and expression level of BCAS1 in several squamous cell carcinoma cell lines, normal keratinocytes and primary tumors.
Methods : We quantitated BCAS1 gene by real-time polymerase chain reaction (PCR). Expression level of BCAS1 was measured by real-time reverse transcription-PCR and immunoblot.
Results : Seven (88%) of 8 squamous cell carcinoma cell lines showed copy number gain of BCAS1 with various degrees. BCAS1 gene in primary tumors (73%) also showed copy number gain. However, expression level did not show a linear correlation with copy number changes.
Conclusions : We identified copy number gain of BCAS1 in squamous cell carcinomas. Due to lack of linear correlation between copy numbers of BCAS1 and its expression level, we could not confirm that the overexpression of BCAS1 is a common finding in squamous cell carcinoma cell lines. However, this study shows that the copy number gain of BCAS1 is a common finding in squamous cell carcinomas.
키워드
Gene dosage; DNA copy number variations; BCAS1 protein; human; Gene expression; Carcinoma; squamous cell
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