The Histone Acetyltransferase hMOF is Overexpressed in Non-small Cell Lung Carcinoma
송준선, 전성민, 이지영, 장세진, 김동관, 김용희,
소속 상세정보
송준선 ( Song Joon-Seon )
University of Ulsan College of Medicine Department of Pathology
전성민 ( Chun Sung-Min )
University of Ulsan College of Medicine Department of Pathology
이지영 ( Lee Ji-Young )
University of Ulsan College of Medicine Department of Pathology
장세진 ( Jang Se-Jin )
University of Ulsan College of Medicine Department of Pathology
김동관 ( Kim Dong-Kwan )
University of Ulsan College of Medicine Department of Thoracic and Cardiovascular Surgery
김용희 ( Kim Yong-Hee )
University of Ulsan College of Medicine Department of Thoracic and Cardiovascular Surgery
KMID : 0357920110450040386
Abstract
Background: One of the histone acetyltransferases (HATs) family of proteins, human MOF (hMOF, MYST1), is involved in histone H4 acetylation, particularly at lysine 16 (H4K16Ac), an epigenetic mark of active genes. Dysregulation of the epigenetic mark influences cellular biology and possibly leads to oncogenesis. We examined the involvement of hMOF and H4K16Ac in primary non-small cell lung cancer (NSCLC).
Methods: Reverse transcription polymerase chain reaction using fresh-frozen lung cancer tissues and lung cancer cell lines and immunohistochemistry for hMOF and H4K16Ac via tissue microarray of 551 formalin-fixed paraffin-embedded NSCLC tissue blocks were conducted.
Results: hMOF mRNA was frequently overexpressed in lung cancer tissues, compared with normal lung tissues (10/20, 50%). NSCLC tissues were positive for hMOF in 37.6% (184/489) and H4K16Ac in 24.7% (122/493) of cases. hMOF protein expression was tightly correlated with the H4K16Ac level in tumors (p<0.001). Knockdown of hMOF mRNA with siRNA led to a significant inhibition of growth in the Calu-6 cell line.
Conclusions: hMOF was frequently expressed in NSCLC and was correlated with H4K16Ac. To our knowledge, this is the first study that has focused on the expression status of HATs and hMOF in NSCLC. Our results clearly suggest a potential oncogenic role of the gene and support its utility as a potential therapeutic target.
키워드
Carcinoma;non-small cell lung;Histones;Acetylation;Histone acetyltransferases
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