TNF-α and TNF-β Polymorphisms are Associated with Susceptibility to Osteoarthritis in a Korean Population
Han Lin, 송주현, 윤정환, 박용규, 이석우, 최유진, 남석우, 이정용, 박원상,
소속 상세정보
( Han Lin )
Catholic University College of Medicine Department of Pathology
송주현 ( Song Joo-Hyoun )
Catholic University College of Medicine Department of Orthopedics
윤정환 ( Yoon Jung-Hwan )
Catholic University College of Medicine Department of Pathology
박용규 ( Park Yong-Gyu )
Catholic University College of Medicine Department of Biostatistics
이석우 ( Lee Suk-Woo )
Catholic University College of Medicine Department of Pathology
최유진 ( Choi Yoo-Jin )
Catholic University College of Medicine Department of Pathology
남석우 ( Nam Suk-Woo )
Catholic University College of Medicine Department of Pathology
이정용 ( Lee Jung-Young )
Catholic University College of Medicine Department of Pathology
박원상 ( Park Won-Sang )
Catholic University College of Medicine Department of Pathology
KMID : 0357920120460010030
Abstract
Background: The tumor necrosis factor (TNF) is believed to play an important role in the pathophysiology of osteoarthritis (OA). Evidence shows that genetic polymorphisms make substantial contributions to the etiology of OA.
Methods: We investigated the genotypes TNF-α and TNF-β in 301 OA patients and 291 healthy subjects as controls. We employed a polymerase chain reaction- restriction fragment length polymorphism and a polymerase chain reaction-single strand conformation polymorphism assay to identify the genotypes TNFA -G308A and TNFB +G252A, respectively.
Results: For TNFA -G308A, the percentages of genotypes GG, AG, and AA were 26.3% (79/301), 62.5% (188/301), and 11.3% (34/301) in OA patients and 88.7% (258/291), 11.3% (33/ 291), and 0% (0/291) in controls. For TNFB +G252A, the percentages of genotypes GG, AG, and AA were 15.3% (46/301), 41.9% (126/301), and 42.9% (129/301) in OA patients and 12% (35/291), 52.6% (153/291), and 35.4% (103/291) in controls. There were significant differences in genotypes and alleles of TNFA -308 between OA patients and controls (p<0.0001) and in alleles of TNFB +252 (p=0.0325). The risk of OA was significantly higher for carriers of the TNFA -308A allele and the TNFB +252 AA homozygote (p=0.0224).
Conclusions: The results suggest close relationships between TNFA -G308A and TNFB +G252A polymorphisms and individual susceptibility to OA in the Korean population.
키워드
Genetic polymorphism; Genetic predisposition to disease; Osteoarthritis; Tumor necrosis factor-alpha; Lymphotoxin-alpha
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