Detection of BRAFV600E Mutations in Papillary Thyroid Carcinomas by Peptide Nucleic Acid Clamp Real-Time PCR: A Comparison with Direct Sequencing
정동준, 정유준, Lee Sung-Che, 이혜란, Lee Wan-Ju, 김형주, 박두산, 박소영, Mu Wenxia, 조현덕, 오미혜, 이성수, 양승하, 김창진,
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정동준 ( Jeong Dong-Jun )
Soonchunhyang University College of Medicine Department of Pathology
정유준 ( Jeong Yu-Jun )
Soonchunhyang University College of Medicine Department of Pathology
( Lee Sung-Che )
Soonchunhyang University College of Medicine Department of Pathology
이혜란 ( Lee Hye-Ran )
Soonchunhyang University College of Medicine Department of Pathology
( Lee Wan-Ju )
Soonchunhyang University College of Medical Sciences
김형주 ( Kim Hyung-Joo )
Soonchunhyang University College of Medicine Department of Pathology
박두산 ( Park Doo-San )
Soonchunhyang University College of Medicine Department of Pathology
박소영 ( Park So-Young )
Soonchunhyang University College of Medicine Department of Pathology
( Mu Wenxia )
Soonchunhyang University College of Medicine Department of Pathology
조현덕 ( Cho Hyun-Deuk )
Soonchunhyang University College of Medicine Department of Pathology
오미혜 ( Oh Mee-Hye )
Soonchunhyang University College of Medicine Department of Pathology
이성수 ( Lee Sung-Soo )
Soonchunhyang University College of Medicine Department of Preventive Medicine
양승하 ( Yang Seung-Ha )
Soonchunhyang University College of Medicine Department of Pathology
김창진 ( Kim Chang-Jin )
Soonchunhyang University College of Medicine Department of Pathology
KMID : 0357920120460010061
Abstract
Background: Papillary thyroid carcinoma (PTC) of the thyroid is the most common endocrine malignancy. High prevalence of an activating point mutation of BRAF gene, BRAFV600E, has been reported in PTC. We assessed the efficiency of peptide nucleic acid clamp real-time polymerase chain reaction (PNAcqPCR) for the detection of BRAFV600E mutation in PTC in comparison with direct sequencing (DS).
Methods: A total of 265 thyroid lesions including 200 PTCs, 5 follicular carcinomas, 60 benign lesions and 10 normal thyroid tissues were tested for BRAFV600E mutation by PNAcqPCR and DS.
Results: The sensitivity and accuracy of the PNAcqPCR method were both higher than those of DS for the detection of the BRAFV600E mutation. In clinical samples, 89% of PTCs harbored the BRAFV600E mutation, whereas 5 follicular carcinomas, 50 benign lesions and 10 normal thyroid tissues lacked the mutation. The mutation was associated with aggressive clinical behaviors as extrathyroid invasion (p=0.015), lymph node metastasis (p=0.002) and multiple tumor numbers (p=0.016) with statistical significance.
Conclusions: The PNAcqPCR method is efficiently applicable for the detection of the BRAFV600E mutation in PTCs in a clinical setting.
키워드
Thyroid; Thyroid cancer; papillary; BRAF; Peptide nucleic acids
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