Cyclooxygenase-2 Expression and Its Prognostic Significance in Clear Cell Renal Cell Carcinoma
이지원, 박정환, Suh Ja-Hee, 남경한, 최지영, 정혜연, Chae Ji-Yoen, 문경철,
소속 상세정보
이지원 ( Lee Ji-Won )
Seoul National University School of Medicine Department of Pediatrics
박정환 ( Park Jeong-Hwan )
Seoul National University College of Medicine Department of Pathology
( Suh Ja-Hee )
Seoul National University College of Medicine Department of Pathology
남경한 ( Nam Kyung-Han )
Seoul National University College of Medicine Department of Pathology
최지영 ( Choe Ji-Young )
Seoul National University College of Medicine Department of Pathology
정혜연 ( Jung Hae-Yoen )
Seoul National University College of Medicine Department of Pathology
( Chae Ji-Yoen )
Seoul National University College of Medicine Department of Pathology
문경철 ( Moon Kyung-Chul )
Seoul National University College of Medicine Department of Pathology
KMID : 0357920120460030237
Abstract
Background : The prognostic value of cyclooxygenase-2 (COX-2) in human renal cell carcinoma (RCC) remains unclear. The purposes of this study are to elucidate the clinical significance of COX-2 in clear cell RCC (CCRCC) and to assess the treatment effect of COX-2 inhibition on CCRCC cell lines.
Methods : Using tumor samples obtained from 137 patients who had undergone nephrectomy at Seoul National University Hospital, we evaluated COX-2 expression on immunohistochemistry. Moreover, we performed the cell proliferation assay using 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-2H tetrazolium bromide (MTT) and cell invasion assay. Thus, we evaluated the effect of meloxicam, an inhibitor of COX-2, in two human CCRCC cell lines.
Results : Cancer-specific survival (p=0.038) and progression-free survival (p=0.031) were shorter in the COX-2 high expression group. A multivariate logistic regression model showed that COX-2 expression was an independent risk factor for pTNM stage and Fuhrman nuclear grade. The MTT assay revealed that COX-2 inhibition led to the suppression of the proliferation of CCRCC cell lines. Moreover, it also reduced their invasion capacity.
Conclusions : This study postulates that COX-2 is a poor prognostic indicator in human CCRCC, suggesting that COX-2 inhibition can be a potential therapy in CCRCC.
키워드
Carcinoma; renal cell; Cyclooxygenase 2; Prognos
원문 및 링크아웃 정보
등재저널 정보