Construction of High-Density Tissue Microarrays at Low Cost by Using Self-Made Manual Microarray Kits and Recipient Paraffin Blocks
최창환, 김규호, 송주영, 최석진, 김루시아, 박인서, 한지영, 김준미, 주영채,
소속 상세정보
최창환 ( Choi Chang-Hwan )
Inha University College of Medicine Department of Pathology
김규호 ( Kim Kyu-Ho )
Inha University College of Medicine Department of Pathology
송주영 ( Song Ju-Young )
Inha University College of Medicine Department of Pathology
최석진 ( Choi Suk-Jin )
Inha University College of Medicine Department of Pathology
김루시아 ( Kim Lucia )
Inha University College of Medicine Department of Pathology
박인서 ( Park In-Suh )
Inha University College of Medicine Department of Pathology
한지영 ( Han Jee-Young )
Inha University College of Medicine Department of Pathology
김준미 ( Kim Joon-Mee )
Inha University College of Medicine Department of Pathology
주영채 ( Chu Young-Chae )
Inha University College of Medicine Department of Pathology
KMID : 0357920120460060562
Abstract
Background: Advances of tissue microarray (TMA) technology have enabled simultaneous in situ analysis of biomarker expression in a large number of archived pathology specimens. However, the relatively high cost of TMA construction may hamper many researchers from using this essential tool of modern pathology research. We discuss methods for making TMA kits and recipient blocks for manual construction of high-density TMAs at low cost.
Methods: Ordinary cannula piercing needles, hypodermic needles, bone marrow biopsy needles, metallic ink cartridges of ballpoint pens, and disposable skin biopsy punches were used to construct self-made manual TMA kits. The recipient blocks were manufactured by boring holes in the conventional bare paraffin blocks. A mini electric hand drill and a microcompound table assembled on a drill stand were used to maximize the capacity of the recipient blocks.
Results: By using TMA kits made from cannula piercing needles (16- and 18-gauge), it was possible to construct TMAs with 1 mm×140 cores, 0.6 mm×320 cores, 2 mm×70 cores, 3 mm×35 cores, and 5 mm×12 cores. The capacity of the recipient blocks could be dramatically increased by drilling holes.
Conclusions:Construction of TMAs using self-made TMA kits is an inexpensive alternative to construction of TMAs using commercial devices.
키워드
Tissue array analysis; Paraffin; Pathology; Research
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