No Detection of Simian Virus 40 in Malignant Mesothelioma in Korea
엄민섭, Abdul Ghafar Jamshid, 박선미, 한정호, 홍순원, 권건영, 고은숙, 김루시아, 김완섭, 하승연, 이교영, 이창훈, 윤혜경, 최유덕, 정명자, 정순희,
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엄민섭 ( Eom Min-Seob )
Yonsei University Wonju College of Medicine Department of Pathology
( Abdul Ghafar Jamshid )
Yonsei University Wonju College of Medicine Department of Pathology
박선미 ( Park Sun-Mi )
Yonsei University Wonju College of Medicine Department of Pathology
한정호 ( Han Joung-Ho )
Sungkyunkwan University School of Medicine Samsung Medical Center Department of Pathology
홍순원 ( Hong Soon-Won )
Yonsei University College of Medicine Department of Pathology
권건영 ( Kwon Kun-Young )
Keimyung University College of Medicine Department of Pathology
고은숙 ( Ko Eun-Suk )
Soonchunhyang University College of Medicine Department of Pathology
김루시아 ( Kim Lucia )
Inha University College of Medicine Department of Pathology
김완섭 ( Kim Wan-Seop )
Konkuk University College of Medicine Department of Pathology
하승연 ( Ha Seung-Yeon )
Gachon University of Medicine and Science Gil Medical School Department of Pathology
이교영 ( Lee Kyo-Young )
Catholic University College of Medicine Department of Pathology
이창훈 ( Lee Chang-Hun )
Pusan National University School of Medicine Department of Pathology
윤혜경 ( Yoon Hye-Kyoung )
Inje University College of Medicine Department of Pathology
최유덕 ( Choi Yoo-Duk )
Chonnam National University Medical School Department of Pathology
정명자 ( Chung Myoung-Ja )
Chonbuk National University Medical School Department of Pathology
정순희 ( Jung Soon-Hee )
Yonsei University Wonju College of Medicine Department of Pathology
KMID : 0357920130470020124
Abstract
Background: Simian virus 40 (SV40), a polyomavirus, was discovered as a contaminant of a human polio vaccine in the 1960s. It is known that malignant mesothelioma (MM) is associated with SV40, and that the virus works as a cofactor to the carcinogenetic effects of asbestos. However, the reports about the correlation between SV40 and MM have not been consistent. The purpose of this study is to identify SV40 in MM tissue in Korea through detection of SV40 protein and DNA. Methods: We analyzed 62 cases of available paraffin-blocks enrolled through the Korean Malignant Mesothelioma Surveillance System and performed immunohistochemistry for SV40 protein and real-time polymerase chain reaction (PCR) for SV40 DNA. Results: Of 62 total cases, 40 had disease involving the pleura (64.5%), and 29 (46.8%) were found to be of the epithelioid subtype. Immunostaining demonstrated that all examined tissues were negative for SV40 protein. Sufficient DNA was extracted for real-time PCR analysis from 36 cases. Quantitative PCR of these samples showed no increase in SV40 transcript compared to the negative controls. Conclusions: SV40 is not associated with the development of MM in Korea.
키워드
Immunohistochemistry; Mesothelioma; Polymerase chain reaction; Simian virus 40
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