In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks
김규호, 최석진, Choi Yeon-Il, 김루시아, 박인서, 한지영, 김준미, 주영채,
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김규호 ( Kim Kyu-Ho )
Inha University Hospital Department of Pathology
최석진 ( Choi Suk-Jin )
Inha University Hospital Department of Pathology
( Choi Yeon-Il )
Inha University Hospital Department of Pathology
김루시아 ( Kim Lucia )
Inha University Hospital Department of Pathology
박인서 ( Park In-Suh )
Inha University Hospital Department of Pathology
한지영 ( Han Jee-Young )
Inha University Hospital Department of Pathology
김준미 ( Kim Joon-Mee )
Inha University Hospital Department of Pathology
주영채 ( Chu Young-Chae )
Inha University Hospital Department of Pathology
KMID : 0357920130470030238
Abstract
Background: Self-made tissue punches can be effectively used to punch holes in blank recipient paraffin blocks and extract tissue cores from the donor paraffin blocks for the low-cost construction of tissue microarrays (TMAs). However, variable degrees of section distortion and loss of the tissue cores can occurs during cutting of the TMAs, posing technical problems for in-house manual construction of high-density TMAs. We aimed to update the method for in-house manual TMA construction to improve the quality of high-density TMAs. Methods: Blocks of agarose gel were subjected to the standard tissue processing and embedding procedure to prepare recipient agarose-paraffin blocks. The self-made tissue punches and recipient agarose-paraffin blocks were used to construct TMAs, which were completely melted and re-embedded in paraffin to make finished TMA blocks. Results: The donor tissue cores were completely integrated into the surrounding paraffin of the recipient blocks. This method enabled us to construct high-density TMAs with significantly less section distortion or loss of tissue cores during microtomy. Conclusions: Simple and inexpensive construction of high-density and high-quality TMAs can be warranted by using paraffinized agarose gels as recipient blocks.
키워드
Tissue array analysis; Sepharose; Paraffin; Pathology; Research
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