Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath
이연수, 공경엽, 손진희, 류기성, 이정헌, 강신광, 조경자, 김용만, 강창석,
소속 상세정보
이연수 ( Lee Youn-Soo )
Catholic University College of Medicine Department of Hospital Pathology
공경엽 ( Gong Gyung-Yub )
University of Ulsan College of Medicine Asan Medical Center Department of Pathology
손진희 ( Sohn Jin-Hee )
Sungkyunkwan University School of Medicine Kangbuk Samsung Hospital Department of Pathology
류기성 ( Ryu Ki-Sung )
Catholic University College of Medicine Department of Obstetrics and Gynecology
이정헌 ( Lee Jung-Hun )
Sungkyunkwan University School of Medicine Kangbuk Samsung Hospital Department of Obstetrics and Gynecology
강신광 ( Khang Shin-Kwang )
University of Ulsan College of Medicine Asan Medical Center Department of Pathology
조경자 ( Cho Kyung-Ja )
University of Ulsan College of Medicine Asan Medical Center Department of Pathology
김용만 ( Kim Yong-Man )
University of Ulsan College of Medicine Asan Medical Center Department of Obstetrics and Gynecology
강창석 ( Kang Chang-Suk )
Catholic University College of Medicine Department of Hospital Pathology
KMID : 0357920130470030265
Abstract
Background: The objective of this study was to evaluate a newly-developed EASYPREP liquidbased cytology method in cervicovaginal specimens and compare it with SurePath. Methods: Cervicovaginal specimens were prospectively collected from 1,000 patients with EASYPREP and SurePath. The specimens were first collected by brushing for SurePath and second for EASYPREP. The specimens of both methods were diagnosed according to the Bethesda System. Additionally, we performed to REBA HPV-ID genotyping and sequencing analysis for human papillomavirus (HPV) on 249 specimens. Results: EASYPREP and SurePath showed even distribution of cells and were equal in cellularity and staining quality. The diagnostic agreement between the two methods was 96.5%. Based on the standard of SurePath, the sensitivity, specificity, positive predictive value, and negative predictive value of EASYPREP were 90.7%, 99.2%, 94.8%, and 98.5%, respectively. The positivity of REBA HPV-ID was 49.4% and 95.1% in normal and abnormal cytological samples, respectively. The result of REBA HPV-ID had high concordance with sequencing analysis. Conclusions: EASYPREP provided comparable results to SurePath in the diagnosis and staining quality of cytology examinations and in HPV testing with REBA HPV-ID. EASYPREP could be another LBC method choice for the cervicovaginal specimens. Additionally, REBA HPV-ID may be a useful method for HPV genotyping.
키워드
Cytological techniques; Vaginal smears; EASYPREP; Human papillomavirus; REBA HPV-ID
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