ERG Immunohistochemistry as an Endothelial Marker for Assessing Lymphovascular Invasion
김세헌, 박형규, 정호영, 이소영, 민경환, 김욱연, 한혜승, 김완섭, 황태숙, 임소덕,
소속 상세정보
김세헌 ( Kim Se-Hun )
Konkuk University Medical Center Department of Pathology
박형규 ( Park Hyung-Kyu )
Konkuk University Medical Center Department of Pathology
정호영 ( Jung Ho-Young )
Konkuk University Medical Center Department of Pathology
이소영 ( Lee So-Young )
Konkuk University Medical Center Department of Pathology
민경환 ( Min Kyueng-Whan )
Konkuk University Medical Center Department of Pathology
김욱연 ( Kim Wook-Youn )
Konkuk University Medical Center Department of Pathology
한혜승 ( Han Hye-Seung )
Konkuk University Medical Center Department of Pathology
김완섭 ( Kim Wan-Seop )
Konkuk University Medical Center Department of Pathology
황태숙 ( Hwang Tae-Sook )
Konkuk University Medical Center Department of Pathology
임소덕 ( Lim So-Dug )
Konkuk University Medical Center Department of Pathology
KMID : 0357920130470040355
Abstract
Background: ERG, a member of the ETS family of transcription factors, is a highly specific endothelial marker. We investigated whether the use of ERG immunostaining can help pathologists detect lymphovascular invasion (LVI) and decrease interobserver variability in LVI diagnosis. Methods: Fifteen cases of surgically resected colorectal cancers with hepatic metastasis were selected and the most representative sections for LVI detection were immunostained with ERG, CD31, and D2-40. Eight pathologists independently evaluated LVI status on hematoxylin and eosin (H&E) and the corresponding immunostained sections and then convened for a consensus meeting. The results were analyzed by kappa (κ) statistics. Results: The average rate of LVI positivity was observed in 43% with H&E only, 10% with CD31, 29% with D2-40, and 16% with ERG. Agreement among pathologists was fair for H&E only (κ=0.27), D2-40 (κ=0.21), ERG (κ=0.23), and was moderate for CD31 (κ=0.55). Consensus revealed that ERG nuclear immunoreactivity showed better visual contrast of LVI detection than the other staining, with improved agreement and LVI detection rate (κ=0.65, LVI positivity rate 80%). Conclusions: The present study demonstrated a superiority with ERG immunostaining and indicated that ERG is a promising panendothelial marker that might help pathologists increase LVI detection and decrease interobserver variability in LVI diagnosis.
키워드
Lymphovascular invasion; Endothelial marker; Immunohistochemistry; ERG; CD31
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