Immunohistochemical Classification of Primary and Secondary Glioblastomas
이규상, 최기영, 남경한, 서안나, 윤수미, 김경주, 조화진, 박성혜,
소속 상세정보
이규상 ( Lee Kyu-Sang )
Seoul National University College of Medicine Department of Pathology
최기영 ( Choe Ghee-Young )
Seoul National University Bundang Hospital Department of Pathology
남경한 ( Nam Kyung-Han )
Seoul National University Bundang Hospital Department of Pathology
서안나 ( Seo An-Na )
Seoul National University Bundang Hospital Department of Pathology
윤수미 ( Yun Su-Mi )
Seoul National University Hospital Department of Pathology
김경주 ( Kim Kyung-Ju )
Seoul National University Hospital Department of Pathology
조화진 ( Cho Hwa-Jin )
Seoul National University Hospital Department of Pathology
박성혜 ( Park Sung-Hye )
Seoul National University Hospital Department of Pathology
KMID : 0357920130470060541
Abstract
Background: Glioblastomas may develop de novo (primary glioblastomas, P-GBLs) or through progression from lower-grade astrocytomas (secondary glioblastomas, S-GBLs). The aim of this study was to compare the immunohistochemical classification of glioblastomas with clinically determined P-GBLs and S-GBLs to identify the best combination of antibodies for immunohistochemical classification.
Methods: We evaluated the immunohistochemical expression of epidermal growth factor receptor (EGFR), p53, and isocitrate dehydrogenase 1 (IDH-1) in 150 glioblastoma cases.
Results: According to clinical history, the glioblastomas analyzed in this study consisted of 146 P-GBLs and 4 S-GBLs. Immunohistochemical expression of EGFR, p53, and IDH-1 was observed in 62.6%, 49.3%, and 11.1%, respectively. Immunohistochemical profiles of EGFR(+)/ p53(?), IDH-1(?)/EGFR(+)/p53(?), and EGFR(?)/p53(+) were noted in 41.3%, 40.2%, and 28.7%, respectively. Expression of IDH-1 and EGFR(?)/p53(+) was positively correlated with young age. The typical immunohistochemical features of S-GBLs comprised IDH-1(+)/EGFR(?)/p53(+), and were noted in 3.6% of clinically P-GBLs. The combination of IDH-1(?) or EGFR(+) was the best set of immunohistochemical stains for identifying P-GBLs, whereas the combination of IDH-1(+) and EGFR(?) was best for identifying S-GBLs.
Conclusions: We recommend a combination of IDH-1 and EGFR for immunohistochemical classification of glioblastomas. We expect our results to be useful for determining treatment strategies for glioblastoma patients.
키워드
Glioblastoma; Immunohistochemistry; IDH1 protein; human; Genes; erbB-1; Genes; p53
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