Evaluation of Protein Expression in Housekeeping Genes across Multiple Tissues in Rats
김혜정, 나종인, 민병우, 나주영, 이경화, 이제혁, 이영직, 김형석, 박종태,
소속 상세정보
김혜정 ( Kim Hye-Jeong )
Chonnam National University Medical School Department of Forensic Medicine
나종인 ( Na Jong-In )
Chonnam National University Medical School Department of Forensic Medicine
민병우 ( Min Byung-Woo )
Chonnam National University Medical School Department of Forensic Medicine
나주영 ( Na Joo-Young )
Chonnam National University Medical School Department of Forensic Medicine
이경화 ( Lee Kyung-Hwa )
Chonnam National University Medical School Department of Pathology
이제혁 ( Lee Jae-Hyuk )
Chonnam National University Medical School Department of Pathology
이영직 ( Lee Young-Jik )
Chonnam National University Medical School Department of Forensic Medicine
김형석 ( Kim Hyung-Seok )
Chonnam National University Medical School Department of Forensic Medicine
박종태 ( Park Jong-Tae )
Chonnam National University Medical School Department of Forensic Medicine
KMID : 0357920140480030193
Abstract
Background: Housekeeping genes, which show constant protein expression patterns between different tissue types, are very important in molecular biological studies as an internal control for protein research.
Methods: The protein expression profiles of seven housekeeping genes (HPRT1, PPIA, GYS1, TBP, YWHAZ, GAPDH, and ACTB) in various rat tissues (cerebrum, cerebellum, cardiac ventricle and atrium, psoas muscle, femoral muscle, liver, spleen, kidney, and aorta) were analyzed by Western blot and compared by coefficient of variation (CV).
Results: HPRT1 was stably expressed (CV≤10%) in six tissues (cerebrum, cerebellum, ventricle, femoral muscle, spleen, and kidney), PPIA was stably expressed in five tissues (cerebrum, cerebellum, ventricle, spleen and kidney), YWHAZ was stably expressed in three tissues (cerebrum, cerebellum, and kidney), and GAPDH was stably expressed in four tissues (cerebrum, ventricle, psoas muscle, and kidney). In comparison, GYS1, TBP, and ACTB were found to have CV values over 10% in all tissues. Of the seven genes examined, four (HPRT1, PPIA, YWHAZ, and GAPDH) were found to be stably expressed across multiple organs, with low CV values (≤10%).
Conclusions: These results will provide fundamental information regarding internal controls for protein expression studies and can be used for analysis of postmortem protein degradation patterns in forensic medicine.
키워드
Genes; essential; Proteins; Tissues; Rats; Postmortem changes
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