Preparation of Compact Agarose Cell Blocks from the Residues of Liquid-Based Cytology Samples
최석진, Choi Yeon-Il, 김루시아, 박인서, 한지영, 김준미, 주영채,
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최석진 ( Choi Suk-Jin )
Inha University School of Medicine Inha University Hospital Department of Pathology
( Choi Yeon-Il )
Inha University School of Medicine Inha University Hospital Department of Pathology
김루시아 ( Kim Lucia )
Inha University School of Medicine Inha University Hospital Department of Pathology
박인서 ( Park In-Suh )
Inha University School of Medicine Inha University Hospital Department of Pathology
한지영 ( Han Jee-Young )
Inha University School of Medicine Inha University Hospital Department of Pathology
김준미 ( Kim Joon-Mee )
Inha University School of Medicine Inha University Hospital Department of Pathology
주영채 ( Chu Young-Chae )
Inha University School of Medicine Inha University Hospital Department of Pathology
KMID : 0357920140480050351
Abstract
Background: Inevitable loss of diagnostic material should be minimized during cell block preparation. We introduce a modified agarose cell block technique that enables the synthesis of compact cell blocks by using the entirety of a cell pellet without the loss of diagnostic material during cell block preparations. The feasibility of this technique is illustrated by high-throughput immunocytochemistry using high-density cell block microarray (CMA).
Methods: The cell pellets of Sure-Path residues were pre-embedded in ultra-low gelling temperature agarose gel and re- embedded in standard agarose gel. They were fixed, processed, and embedded in paraffin using the same method as tissue sample processing. The resulting agarose cell blocks were trimmed and represented on a CMA for high-throughput analysis using immunocytochemical staining.
Results: The SurePath residues were effectively and entirely incorporated into compact agarose cell buttons and embedded in paraffin. Sections of the agarose cell blocks revealed cellularities that correlated well with corresponding SurePath smears and had immunocytochemical features that were sufficient for diagnosis of difficult cases.
Conclusions: This agarose-based compact cell block technique enables preparation of high-quality cell blocks by using up the residual SurePath samples without loss of diagnostic material during cell block preparation.
키워드
Immunocytochemistry; Biopsy; fine-needle; Paraffin embedding; Sepharose; Cell block microarray
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