Escherichia coli에서의 L-asparaginase부분정제 및 그 특성에 관한 연구
Partial Purification of the L-Asparaginase from Escherichia Coli and a Study on its Enzymatic Properties
소속 상세정보
박노정/Pak RJ
KMID : 0358319730140030155
Abstract
1. Based on the differences in solubility in ammonium sulfate and activity as a function of pH, two L-asparaginases, EC-1 and 2, were partially purified from Escherichia coli 0112. 2. Both L-asparaginases, EC-1 and 2, were highly activated with low concentration of their substrate, L-aspartgine, but inactivated with high concentration of it. 3. Activities of L-asparaginases, EC-1 and 2, were inhibited with the product, L-aspartic acid in proportion to its concentration 4. Both of them were denatured by urea, EC-1 being denatured completely with 6M and EC-2 with 8M urea, showing their spatial conformational difference, since the latter proved to be a little more resistant to urea than the former.
키워드
세균학; 대장균; l-Asparaginase; E. coli
원문 및 링크아웃 정보
등재저널 정보