종양괴사인자 유전자 이입 방광암세포주 이식이 C3H/He 생쥐의 생존에 미치는 영향
Effect of Tumor Necrosis Factor-alpha Gene Transduced Bladder Tumor Cell Xenograft on Survival of C3H/He Mice
주인온, 이은식, 김계희,
소속 상세정보
주인온 ( )
서울대학교
이은식 ( )
서울대학교
김계희 ( )
서울대학교
KMID : 0358319960370121333
Abstract
Objective:
@EN To evaluate the effect of tumor necrosis factor-α (TNF-α) gene transduced bladder tumor cells on survival of C3H/He mice.
@ES Materials and Methods:
@EN The complete cDNA of TNF-α was introduced to mouse bladder tumor cell line(MBT-2) using a retroviral vector. Gene transfer and expression were confirmed by polymerase chain reactijon9PCR0 and reverse transcription polymerase chain
reaction9RT-PCR).
Produced TNF-α was measured by ELISA. TNF-α gene transduced and parental cells were xenografted in C3H/He mice. Implantation and growth rate and survial of experimental animals were analysed.
@ES Results:
@EN Successful gene transfer and expression were confirmed. TNF-α gene transduced MBT-2 cells secreted TNF-α at the concentration of 47.4pg/10E6cells/24hours. The implantation and growth rate of TNF-α gene transduced MBT-2 cells much lower
than
those
of parental cells and survival of experimental animals was different between TNF-α gene transduced MBT-2 and parental cells(P<0.05).
@ES conclusion:
@EN This study shows potential advantages of localized TNF-α secretion to induce potent antitumorigenic response. Tumor cell targeted TNF-α gene therapy could become a useful tool in treatment of bladder cancer.
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