이입된 종양괴사인자 유전자발현이 인체방광암세포주의 성장에 미치는 영향
Effect of Transduced Tumor Necrosis Factor-alpha Gene Experession on Growth of Human Bladder Tumor Cell Lines
이해원, 이은식, 김계희,
소속 상세정보
이해원 ( )
서울대학교
이은식 ( )
서울대학교
김계희 ( )
서울대학교
KMID : 0358319960370121339
Abstract
Objective:
@EN To evaluate the effect of transduced tumor necrosis factor-α(TNF-α) gene expression on growth of human bladder tumor cell lines in vitro.
@ES materials and Methods:
@EN The complete cDNA of TNF-α was introduced to three human bladder tumor cell lines(T24, J-82, HT-1197) using a retroviral vector, a recombinant form of Molony murine kleukemia virus with TNF-α and Neo gene and transfected cells were selected
by
exposure to neomycin analog G418. Gene transfer and expression were confirmed by polymerase chain reaction9PCR) and reverse transcription polymerase chain reaction(RT-PCR)-Southern blotting. Cell growth was measured by MTT assay.
@ES Results:
@EN Successful gene transfer and expression were confirmed in all three cell bladder tumor lines. Growth of transfectd cells were compared with parental cell lines and no differences were found in all three lines(p>0.05).
@ES Conclusion:
@EN Expression of transduced TNF-α gene could not show any effect on growth of human bladder tumor cells in vitro.
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