Histone MRNA In Situ Hybridization을 통한 N-butyl-N-(4-hydroxybutyl) Nitrosamine (BBN)에 유발된 백서방광종양의 증식양상
Proliferation Patterns of N-butyl-N-(4-hydroxybutyl) Nitrosamine(BBN) Induced Rat Bladder Tumor Identified by Histone MRNA In Situ Hydrdization.
유창우, 서정원, 김용태, 김원재,
소속 상세정보
유창우 ( )
Chungbuk National University
서정원 ( )
Chungbuk National University
김용태 ( )
Chungbuk National University
김원재 ( )
Chungbuk National University
KMID : 0358319970380020111
Abstract
The synthesis of histone mRNA is closely coupled with DNA replication. During the S-phase of the cell cycle, the level of histone mRNA increases over fifity fold then rapidly disappears at the start of G2-phase. The presence, therefore, of
abundant
quantities of histone mRNa provides a molecular of cycling cells.
The expression of histone mRNA was investigated by in situ hybridization method in N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) induced rat bladder tumor to determine the proliferation patterns. This method resulted in intense brown/black
cytoplasmic
staining of cells containing histone mRNA and extent and intensity of stain were increased in tumorigenesis (normal, simple hyperplasia, nodular or papillary hyperplasia, and transitional cell carcinoma-Ta, T1). The higher S-phase fraction
indicated
greater biological malignancy based on the fact that the extent and intensity of stain progressively increased with tumorigenesis.
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