종양괴사인자 유전자이입 방광임 세포주에서 세포성장과 이입된 유전자 발현에 미치는 Retinoic Acid의 영향
Effect of Retinoic Acid on Growth and Transduced Tumor Necrosis Factor-α Gene Expression of Human Bladder Tumor Cell Lines
소속 상세정보
정현
윤상진/조문기/이해원/김수웅/이은식/이종욱
KMID : 0358319970380030229
Abstract
Introduction and Objectives:
@EN Retinoic acid (RA) is known as a potent chemopreventive agent in bladder tumor. Recently, RA has gained attention for upregulation of transduced gene expression via long terminal repeat (LTR) transcriptional promotion. In this study, we
investigated
the possible dual effect of RA, growth inhibition of transduced gene expression which contains LTR promotor in human bladder carcinoma cell lines.
@ES Materials and Methods:
@EN Human bladder carcinoma cell lines (T-24, J-82, HT-1197, ATCC) were transduced with Moloney murine leukemia virus containing cDNA of TNF-α. The growth of transduced and parent cell line was measured by tetrazolium based colorimetric assay
(MTT).
Transduced TNF-α gene expression was determined by ELISA method.
@ES Results:
@EN TNF-α production was increased approximately twofold after treatment with RA(10μM) in all three cell lines. This increase was dependent on RA concentration. RA treatment of transduced and parent cell line resulted in dose dependent
inhibition
of
cell proliferation (up to 80% inhibition with 10μM RA) in all parental and transduced cell lines.
@ES Conclusions:
@EN These results indicate that RA shows dual effect in cytokine gene transduced bladder carcinoma cells with retroviral vector containing LTR promotor and could be a supplement to the gene therapy of bladder cancer.
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