사람정장의 마우스의 체액성 및 세포성 면역반응에 미치는 영향
Effects of Human Seminal Plasma on Humoral and Cellular Immune Response in Mice
이용호, 박영경, 하대유,
소속 상세정보
이용호 ( )
전북대학교
박영경 ( )
전북대학교
하대유 ( )
전북대학교
KMID : 0358319970380040351
Abstract
Although it has been known that human seminal plasma (HSP) suppresses immune responses, there is little data concernning in vivo effects on humoral and cellular immune responses, particularly on immediate hypersensitivity. Thus, the present
study
was
undertaken in an effort to investigate the in vivo effect of HSP on humoral and cellular immune responses, including active systemic anaphylaxis (ASA) in mice. The immune responses investigated were delayed-type hypersensitivity (DTH) reaction to
sheep
red blood cell (SRBC) or human sperm antigen, hemagglutinin response, and active systemic anaphylaxis induced by egg albumin(OVA). Effect of HSP on Candida albicans infection was also studied. It was found that intraperitoneal administration of
HSP
before or after immunization with SRBC significantly suppressed the DTH to SRBC. HSP given after immunization with SRBC failed to suppress hemagglutinin response whereas HSP given before immunization with SRBC significantly suppressed the
hemagglutinin
response, Interestingly, intravaginal administration of HSP together with human sperm significantly suppressed DTH to human sperm as measured by footpad swelling reactions. HSP inhibited phagocytic function of macrophage and enhanced germ tube
producing
phagocytosed yeasts. Colony forming unit(CFU) of Candida albicans I kidneys of HSP-treated mice were enumerated. HSP given to mice before infection significantly increased the number of CFU in kidneys, strongly suggesting that HSP may decrease
the
resistance of mice to Candida infection. For the ASA experiment, mice were sensitized by I.p. injection of 500㎍ OVA, 병원.0mg alum and 대학교×10E9 Bordetella pertussis in 0.의원 ml PBS and were challaned by I.y. injection of 0.25 ml PBS
containing
500㎍ OVA 18 days after sensitization. Suprisingly, HSP injection before ASA induction inhibited intensity of ASA and improved survival of anaphylaxis. Taken together, this study strongly suggests that HSP may suppress in vivo immediate and
delayed
immune responses and that HSP may decrease the resistance against Candida albicans infection, and this study may be the first to show the immunosuppressive effect of HSP on the induction of active systemic anaphylaxis.
키워드
원문 및 링크아웃 정보
등재저널 정보