음경의 침윤성 펀평세포암에서 중합효소 연쇄반응과 조직내 교잡법을 이용한 인형 유두종 바이러스의 검출
Detection of Human Papillomavirus DNA in Invasive Squamous Cell Carcinoma of the Penis Using Polymerase Chain Reaction and in Situ Hybridization
소속 상세정보
박양일/Yangil Park
임정환/오봉렬/류수방/최찬/박창수/Jounghwan Lim/Bongryoul Oh/Soobang Ryu/Chan Choi/Changsoo Park
KMID : 0358319970380080823
Abstract
중합효소 연쇄반응법을 이용한 음경의 침윤성 편평세포암 조직에서 인형유두종 바이러스
DNA가 50%에서 검출되고 모두가 인형유두종 바이러스 DNA type 16 이었다. 이런 결과로
미루어 음경암의 발생에 인형유두종 바이러스 type 16이 중요한 역할을 할 것으로 생각하며
비록 인형유두종 바이러스 DNA type 16과 중복검출 되기는 하였지만 인형유두종 바이러스
DNA type 6/11이 비교적 높은 검출율을 보인 것에 대한 추가적인 연구가 필요할 것으로
생각된다.
#초록#
Purpose: Infection of the external urogenital system with human papillomavirus (HPV)
has been implicated in the development of genital cancer. We evaluated the prevalence
of HPV types 6/11, 16 and 18 deoxyribonucleic acid (DNA) by polymerase chain reaction
(PCR) and the localization of HPV DNA by in situ hybridization.
Material and Method: A total of 22 formalin-fixed, paraffin-embedded specimens of
invasive squamous cell carcinoma of the penis were analyzed. We used the PCR
technique to evaluate type specific DNA sequences of unique E6 to E7 transforming
regions of HPV. Also, we investigated the localization of HPV DNA by in situ
hybridization in PCR positive cases.
Results: Overall, by PCR technique the detection rate for HPV DNA were 50% (11 of
22 cases). HPV DNA type 16 was detected in all positive specimen and type 6/11 in 5
cases, whereas type 18 could not be detected. All of HPV DNA type 6/11 positive
specimens were also HPV DNA type 16 positive. Using in situ hybridization HPV DNA
type 16 was detected in 2 (18.2%) from 11 specimens in which HPV DNA had already
been detected by PCR, and HPV DNA type 16 was localized in the nuclei of scattered
carcinoma cells. But, HPV DNA type 6/11 were not detected by in situ hybridization.
Conclusions: These findings suggest that HPV DNA type 16 is the type most
commonly associated with penile carcinoma. But the result of high detection rate for
HPV DNA type 6/11 seems to require further investigations.
키워드
Penile carcinoma; PCR; In situ hybridization; Invasive; Squamous;
원문 및 링크아웃 정보
등재저널 정보