Allergy성 방광염을 유발시킨 백서에서 Heparin의 방광내주입이 방광점막투과성에 미치는 영향
Effects of Intravesical Heparin Treatment on the Permeability of Bladder Mucosa in Allergic Cystitis in Rat
신현철, 박동춘, 김영수,
소속 상세정보
신현철 ( )
아주대학교 의과대학 비뇨기과학교실
박동춘 ( )
아주대학교 의과대학 비뇨기과학교실
김영수 ( )
영남대학교 의과대학 비뇨기과학교실
KMID : 0358319980390020116
Abstract
결론
Sprague Dawley 암컷 백서 30마리를 대상으로 각각 10마리씩 군에 따른 처치를 한 후,
방광점막의 투과성의 변화를 관찰하기 위하여 각 군에서 연속적으로 말초혈액을 채취하고
마지막 혈액채취 후 방광을 적출하여 각각의 14C-urea를 측정한 결과는 다음
과 같다
1) 생리식염수로 모든 처치를 한 정상 대조군에서는 방광점막의 비투과성이 유지되어 있었
고, 2) 생리식염수 혹은 hepanin으로 방광처치를 한 감작-ovalbumin군 모두에서 방광점막의
투과성이 증가되었으며, 3) hepanin으로 방광내 치료를 한 군이 생리식염수 치료를 한 군
보다 투과성이 낮게 나왔다. 4)그후 방광을 절제하여 방광조직내에 침투된 14
C-urea를 비교한 결과도 위와 동일하게 나왔다.
이상의 결과에서 allergy성 방광염을 유발시킨 백서에서 증가된 방광점막의 투과성을 방광
내 hepanin의 처치에 의해 방광점막의 투과성을 감소시킬 수 있음을 알 수 있었다.
#초록#
Purpose: The relative impermeability of the bladder mucosa is due to the
glycosaminoglycan layer covering the urothelium and the tight junction of the
urothelium. Recently, one of the most popular theories of interstitial cystitis is the
penetration of urinary irritants into the suburothelial tissue due to an increased
permeability of the urothelium. This study was performed to evaluate the effect of the
intravesical heparin treatment on the permeability of bladder mucosa in allergic cystitis.
Materials and Methods: Rats were sensitized by intraperitoneal injection of
ovalbumin(10mg/m1/kg) given on days 1, 3 and 5. The experiments were performed 4
weeks afrer the last injection. Controls were run simultaneously with the sensitized
animals. Sensitized rats were challenged with intravesical ovalbumin(10mg/m1, 1ml) and
control rats received 1 ml saline Intravesically. Sensitized-antigen challenged group was
divided into two subgroups; rats treated with intravesical hepanin(5mg/ml in 0.9% NaCl)
or those treated with 1 ml saline intravesically Immediately following the intravesical
heparin(or saline) treatment, 1ml of 14C-urea was placed into the bladder
for two hours. We examined the peripheral blood concentration of 14C-urea
at periods up to 120 minutes.
Results: There was no 14C-urea present in the blood in control group.
There was a progressive increase in the blood level of 14C-urea with time
in the sensitized-antigen challenge group. Compared with intravesical saline treatment
group, there was less progressive increase in the blood level of 14C-urea
with time in the intravesical heparin treatment group. W8 also measured radioactivity of
14C-urea in the bladder tissues and found significantly lower level of
14C-urea in the bladder tissues from intravesical heparin treatment group
than intravesical saline treatment group.
Conclusions: This study indicates that immunologically induced cystitis increases
bladder mucosal permeability in rats and intravesical heparin treatment decrease the
permeability significantly.
키워드
Cystitis; Bladder mucosal permeability; Intravesical heparin treatment;
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