쥐의 방광출구 폐색시 인터페론 감마 피하주사에 의해 유도된 콜라겐 변화의 형태학적 분석
Morphometric Analysis of the Collagen Changes Induced by Subcutaneous Injection of Interferon-γ following Bladder Outlet Obstruction in the Rat
정정윤, 장선오, 이규성,
소속 상세정보
정정윤 ( )
서울대학교 의과대학 비뇨기과학교실
장선오 ( )
서울대학교 의과대학 비뇨기과학교실
이규성 ( )
성균관대학교 의과대학 비뇨기과학교실
KMID : 0358319980390090844
Abstract
결론
저자들은 방광출구 부분폐색을 유도한 쥐에게 IFN-γ를 투여한 후 방광내 콜라겐 침착의
분포와 양을 형태학적인 방법으로 관찰하여 IFN-γ가 폐색방광에서 콜라겐 침착을 감소시
키는지를 확인하기 위하여 시행되었다. 암컷 성인 Sprague-Dawley쥐에서 방광출구 부분폐
색을 4주간 유지한 군과 방광출구 부분폐색 후 4주간 IFN-γ 100,000단위를 투여한 군에서
콜라겐 침착의 변화를 형태학적인 방법으로 측정하여 다음과 같은 결론을 얻었다.
1 형태학적인 분석 결과, IFN-γ투여는 앙광출구 부분폐색으로 인해 침착이 증가된 콜라
겐을 유의하게 감소시켰다
2. IFN-γ는 가장수술군에 투여한 경우와 폐색군에 투여한 경우 모두에서 콜라겐을 감소
시켰다. 이상의 결과로 IFN-γ투여는 폐색방광에서 방광내 콜라겐 생성을 억제하여 방광유
순도를 증가시키는 효과를 나타낼 수 있을 것으로 생각한다.
#초록#
Purpose: Recent studies of bladder outlet obstruction have provided evidence that poor
bladder compliance is closely related to collagen content in the bladder wall. To decrease
collagen content in the obstructed bladder, we examined the collagen changes by
interferon gamma after creating partial bladder outlet obstruction in the rat.
Experimental studies of interferon-γ(IFN-γ)have been tried to decrease fibrosis in liver
disease. We applied interferon-γ to the obstructed bladder and observed its effect on
collagen changes in the bladder wall by morphometric analysis.
Materials and Methods: In a total of 50 Sprague-Dawley adult female rats(200-
220gm), we created partial bladder outlet obstruction by placing metal rod (diameter 1
㎜). We divided the rats Into 5 groups(n=10 in each group): normal control(N), sham
operation(5), sham operation and IFN-γ4 weeks(S+IFN), bladder outlet obstruction 4
weeks(BOO), and bladder outlet obstruction and IFN-γ4 weeks(BOO+IFN) groups. IFN-
γ(LG Chemical, Korea) was injected daily(100,000IU) for 4 weeks through subcutaneous
route from obstruction day 1 in the injection groups. The rats in each group were
sacrificed at 4 weeks of the experimental day and bladder body was prepared for
Masson's trichrome special staining. With the image analyser, we calculated the percent
collagen area(collagen/collagen muscle) in each bladder.
Results: With obstruction, bladder weights of BOO group and BOO+IFN group were
increased 4.6 and 4.4 times, respectively, compared to S group. The percent collagen
area in each group was 40.7 1.6% in N group, 38.2±2.0% in S group, 26.6±3.8% in
S+IFN group, 19.9±2.5% in BOO group, and 12.4±2.0% in BOO+IFN group(mean士
standard error). There was no significant statistical difference between N group and S
group(p>0.05 by Wilcoxon's rank sum test). On the contrary, there were significant
statistical differences between N group and S+IFN group and between S group and
S+IFN group. Also there was significant statistical difference between BOO group and
BO0+1FN group(p<0.05).
Conclusions: From the above results, IFN-γseems to have the effect to decrease
collagen content in the obstructed rat bladder. However, furthers investigations are
required to evaluate the therapeutic relevance of IFN-γ on bladder outlet obstruction
patients.
키워드
Rat; Bladder outlet obstruction; Interferon-γ Collagen; Morphometric analysis;
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