거세된 토끼에서 남성호르몬투여가 음경의 Nitric Oxide Synthase에 미치는 영향
The Effect of Testosterone on Nitric Oxide Synthase in the Penis of the Rabbit
최영득, 정우식,
소속 상세정보
최영득 ( )
연세대학교 의과대학 비뇨기과학교실
정우식 ( )
이화여자대학교 의과대학 비뇨기과학교실
KMID : 0358319990400010106
Abstract
서론
음경발기는 음경해면체평활근이 이완되고 소공이 팽창되어 음경내압이 증가됨에 따라 유
발된다. 이러한 음경발기는 음경해면체평활근의 긴장력에 의해 조절되는 바, 비아드레날린성
비콜린성 신경전달물질과 해면체 내피세포에서 분비되는 이완물질이 음경발기의 중심인 음
경해면체평활근의 이완에 중요한 역할을 하고 있음이 밝혀지고 있다. nitric oxide(NO)는 평
활근 이완의 생리학적 전령으로 최근 음경해면체평활근 이완의 주 매개체로 알려지면서 음
경의 NO에 대한 남성호르몬 역할이 연구되고 있다. 그러나 현재까지의 보고를 토대로 남성
호르몬이 음경의 NO에 미치는 직접적인 상호관계를 추정하기는 미흡한 실정이다.
본 연구에서는 남성호르몬이 음경의 NO에 미치는 영향을 관찰하고자 성숙한 토끼를 대
상으로 고환제 거로 남성 호르몬 결핍을 유도하고 기간에 따라 남성 호르몬을 보충하여, 남
성호르몬이 음경발기의 주 매개체로 알려진 NO에 미치는 효과를 관찰하고자 하였다.
Purpose: Androgen was thought to be linked to sexual activity in man but its
peripheral action have not been identified precisely. Recently, androgens dependent nature
of nitric oxide synthase(NOS), the enzyme system producing nitric oxide(NO) as the
principal mediator in penile erection, has been studied. The present study was designed
to determine if NO within the penis is regulated by androgen.
Materials and Methods: Effect of testosterone on the penile NO was investigated from
three groups of rabbit; control(a sham operation), castration group(four sub-groups: 1, 2,
3 and 4 weeks after castration) and testosterone replacement group(four sub-groups:
testosterone propionate(10㎎/day) was given subcutaneously from 1, 2, 3 and 4 weeks
after castration for 7 days respectively). Strips of rabbit corpus cavernosum were
isolated and mounted in organ chambers. The relaxation effects were assessed with
electrical field stimulation(EFS) in the precontracted cavernosal muscle strips with
phenylephrine. Penile NOS activity was measured by the rate of
14C-L-arginine-citrulline conversion. Penile tissue was subsequently
stained for the presence of NOS, using a nicotinamide adenine dinucleotide phosphate
diaphorase(NADPH-d) histochemistry.
Results: EFS evoked a relaxation of rabbit corpus cavernosum. A gradual decrease in
the EFS evoked relaxation was recorded, proportional to the duration of being castrated,
which was restored to the level of control by testosterone replacement. After castration,
penile NOS activity was significantly reduced to 51.3%, 43.7%, 37.5%, and 32.7% of the
level of control at 1, 2, 3, 4 weeks. This decrease in penile NOS activity after castration
was also restored by testosterone replacement. Diffusely scattered delicate nerve fibers
showing blue color reaction after NADPH-d histochemical staining were reduced in
castrated cavernosum in comparison with control.
Conclusions: Based on presented data we conclude that testosterone plays a direct role
in penile erection by regulating the activity of NOS within the penis.
키워드
Testosterone; Nitric oxide; Cavernosal smooth muscle; Rabbit;
원문 및 링크아웃 정보
등재저널 정보