Glucocorticoid가 토끼 음경해면체평활근에 미치는 영향
Effect of Glucocorticoid on Rabbit Corpus Cavernosum Smooth Muscle
서은영, 마상열, 박기현,
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서은영 ( )
연세대학교 의과대학 비뇨기과학교실
마상열 ( )
연세대학교 의과대학 비뇨기과학교실
박기현 ( )
연세대학교 의과대학 비뇨기과학교실
KMID : 0358319990400020234
Abstract
결론
glucocorticold가 음경해면체평활근 작용에 미치는 영향을 평가하고 발기부전의 진단 및
치료에 사용되는 음경해면체 내 주사약제들에 미치는 효과를 관찰하고자 토끼의 음경해면체
평활근 절편에서 HCS의 효과를 관찰한 결과, HCS는 안정상태의 해면체평활근에서 수축이
나 이완작용은 없었으나 농도의존적으로 아드레날린성 약물의 작용을 증강시키며, 또한
PHE으로 수축된 평활근절편에서 HCS는 직접적인 이완효과는 없었으나 HCS 10㎍/㎖는
ACh(10-7M), PGE1(10-2M), papaverine(10-8M),
verapamil(10-6M), SNAP(5×10-5M)의 이완력을, HCS 50㎍/㎖
는 ACh(10-7M)과 phentolamine(10-8M)의 이완력을 증가시키
고, HCS 100㎍/㎖는 ACh(10-7M), PGE1(10-2M),
papaverine(10-8M), verapamil(10-6M), SNAP(5×
10-5M), phentolamine(10-8M)의 이완력을 증가시키지 않았다.
Purpose : Glucocorticoid contribute to the pathogenesis of hypertension through
intracellular signals that stimulate vascular smooth muscle contraction. However, some
in vitro and in vivo studies have shown that glucocorticoid has the potential role of
vasorelaxation. Therefore, we tried to investigate the effect of short acting
glucocorticoid(hydrocortisone; HCS) on the isolated rabbit cavernosal smooth muscle for
evaluation of the possibility of using this material as a pharmacoerecting agent.
Materials and Methods : Strips of rabbit corpus cavernosum were mounted in organ
chambers. On the precontracted muscle strips with phenylephrine(PHE; 5×
l0-6M), HCS was treated with increasing concentration from 10㎍/㎖. The
relaxing activity of ACh(10-7M), phentolamine(10-8M),
papaverine(10-8M), verapamil(10-6M),
PGE1(10-2M), SNAP(5×10-5M) were observed with the
preparation of HCS. Depolarization by KCI were observed with HCS to investigate the
relationship of HCS effects to K+.
Results : Pretreatment of muscle strips with low dose of HCS caused
concentration-related increase of a PHE induced contraction. On muscle strips
submaximally precontracted with PHE, HCS(10㎍/㎖ to 100㎍/㎖) showed no relaxations.
Pretreatment of muscle strips with 10㎍/㎖ dose of HCS caused a potentiation of a
relaxation effects of ACh, papaverine, verapamil, SNAP, PGE1 and 50㎍/㎖ dose of HCS
caused a potentiation of a relaxation effects of ACh, phentolamine. However, 100㎍/㎖
dose of HCS did not produce changes of these potentiating responses to relaxation. Also,
HCS did not influenced the depolarization with any concentrations of KCI.
Conclusions : HCS has the potentiating effect of both the PHE-Induced contraction,
and the relaxation of the ACh, phentolamine, papaverine, verapamil, SNAP, PGE1 at
10-50㎍/㎖ concentration on the cavernosal smooth muscle.
키워드
Glucocorticoid; Cavernosal smooth muscle; Rabbit;
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