쥐의 방광출구 부분 폐색 시 인터페론 감마에 의한 방광 내 콜라겐 발현의 변화
Effects of Interferon Gamma on Collagen Expression in the Partially Obstructed Urinary Bladder of Rat
소속 상세정보
이규성/Kyu Sung Lee
김광명/정정윤/이안기/정해일/박철근/김시황/Kwang Myung Kim/Jung Yun Jung/Ahn Kie Lee/Hae Il Cheong/Cheol Keun Park/Shi Whang Kim
KMID : 0358319990400060740
Abstract
결론
암컷 성인 쥐 Sprague-Dawley에서 방광출구 부분 폐색을 4주간 유지한 군과 방광출구
부분 폐색 후 4주간 IFN-γ 100,000 unit를 투여한 군에서 방광 콜라겐 침착의 변화를 면역
조직화학 염색 방법으로 측정하고 콜라겐 유형 Ⅰ과 콜라겐 유형 Ⅲ cDNA probe를 이용한
Northern blotting을 시행하여 콜라겐 유형 Ⅰ과 콜라겐 유형 Ⅲ mRNA발현을 정량분석 한
결과 IFN-γ투여는 면역 조직화학 염색에서 방광출구 부분 폐색으로 침착이 증가한 배뇨근
다발 사이의 콜라겐 유형 Ⅰ과 콜라겐 유형 Ⅲ를 대조군과 비슷한 정도로 감소시켰고,
sham군에 투여한 경우와 폐색군에 투여한 경우 모두에서 Pro α1(Ⅰ) collagen mRNA 및
Pro α1(Ⅲ) collagen mRNA의 발현을 감소시킴을 관찰하였다.
따라서 IFN-γ 투여는 폐색 방광에서 방광 내 콜라겐 발현을 억제하고 그 억제 기전은
부분적으로 전사 단계에 작용함을 알 수 있었다.
Purpose: Recent studies have proved that bladder compliance is closely related to the
collagen content of the bladder. It has been shown through human fibroblast, condrocyte,
and rat myofibroblasts that interferon(IFN)-γ decreased collagen synthesis. The
objective of this study was to investigate the alteration of collagen deposition and gene
expressions in collagen types Ⅰ and Ⅲ by partial bladder outlet obstruction and to
compare the changes during IFN-γ treatment
Materials and Methods: Following 4 weeks of partial bladder outlet obstruction In
200-250gm. SD rats, )he bladder tissues were evaluated for collagen deposition by an
immunohistochemical staining using the polyclonal antibodies and the messenger
ribonucleic acid(myRNA) contents of the collagen type Ⅰ and Ⅲ were evaluated by
Northern hybridization. Recombinant IFN-γ(100,000 units) was administered once a day
for 4 weeks by subcutaneous injection.
Results: In the control bladders, the immunohistochemical staining for type Ⅰ and
type Ⅲ collagen protein showed an intense localization in the lamina propria with
modest localization in the inter-fascicular region, With obstruction type Ⅰ and type Ⅲ
collagen staining intensified, especially in the inter-fascicular region. The IFN-γ
administration reduced the inter-fascicular deposition of collagen type Ⅰ and Ⅲ. There
was an up-regulation of collagen type Ⅰ and Ⅲ gene expression after the obstruction.
In the obstructed bladders pro α1(Ⅰ) and pro α1(Ⅲ) collagen mRNA intensity was
increased 3.4 and 5.1 folds than the control groups, respectively. This up-regulation was
down-regulated by IFN-γ administration.
Conclusions: The down regulation of collagen types Ⅰ and Ⅲ gene expression, and
decreased protein deposition after IFN-γ treatment demonstrated that the effect of IFN-
γ was, in parti, transcriptionally regulated. These results suggest that the recombinant
IFN-γ may be beneficial in the treatment of patients with poorly compliant bladder.
키워드
Urinary bladder; Collagen; Interferon-γ; Bladder outlet obstruction;
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