고환에서 정세포 증식 및 세포고사가 조정기능에 미치는 영향
The Influence of Germ Cell Proliferation and Apoptosis on Spermatogenesis in Testes
손동완, 박남철, 윤혜경,
소속 상세정보
손동완 ( )
부산대학교 의과대학 비뇨기과학교실
박남철 ( )
부산대학교 의과대학 비뇨기과학교실
윤혜경 ( )
인제대학교 의과대학 병리학교실
KMID : 0358319990400060765
Abstract
결론
불임을 주소로 내원한 고환 부전에 의한 무정자증 환자 58례(불임군)와 임신의 과거력이
있으며 적출된 고환 생검 조직이 정상 정자형성 소견을 가진 전립선암 환자 13례(대조군)에
서 채취된 파라핀포매 고환생검 조직 절편을 연구재료로 하여 MIB-1 발현, bcl-2 발현은
면역조직화학적 염색으로, 세포고사는 TUNEL법으로 분석하여 다음과 같은 결론을 얻었다.
정세포 증식능이 고환에서 조정기능을 반영하지 않았으며, 세포고사는 Sertoli cell only
syndroms을 제외한 조정기능장애를 나타낸 모든 남성불임 환자의 고환조직에서 관찰됨으로
써 정세포의 세포고사와 남성불임과의 연관성을 시사하였다. 향후 정자 형성 과정에서 정세
포의 단계별 성숙과 증식을 지배하는 인자의 발견과 정상적으로 수반되는 세포고사 발현과
억제인자와의 상관관계에 대한 추가적 연구가 필요할 것으로 생각한다.
Purpose: The germ cell proliferation and apoptosis were investigated in testis and its
effect to the spermatogenesis.
Materials and Methods: The biopsy specimens of testis were obtained from 58 infertile
males with nonobstructive azoospermia due to primary testicular failure and from 13
prostate cancer cases with normal spermatogenesis. The infertility specimens were
divided into 4 groups according to histopathologic findings-Sertoli cell only syndrome(A),
maturation arrest(B), hypospermatogenesis (C) and disorganization with sloughing(D) and
quantitatively evaluated with Johnson score system. Germ cell proliferation and apoptosis
were determined using immunohistochemical study with MIB-1, bel-2 monclonal
antibodies and TUNEL method, and evaluated the relationship between their results and
histopathologic findings.
Results: MIB-1 expression was negative in all infertility group A. MIB-1 positivity
was revealed no statistical significance among infertility groups B, C, D and con)rot
group(p>0.05 in each). Bcl-2 and apoptosis were not expressed in all infertilty group A
and control group. Bcl-2 and apoptosis positivity were revealed no significant difference
among group B, C and D(p>0.05). MIB-1 expression was shown no relationship with
the apoptosis(p>0.05), and a high prevalence in bel-2 positive group, but there was no
significant correlation(p>0.05). In infertility group, the MIB-1 index and bel-2 positivity
were decreased according to high Johnson score but no statistical difference(p>0.05), and
apoptosis was shown no relationship with Johnson score(p>0.05).
Conclusions: MIB-1 expression did not represent the degree of spermatogenic failure
In infertile patients. The apoptosis was detected in all infertility groups except Sertoli
cell only syndrome. Apoptosis was suggested to be related somewhat with
spermatogeneis.
키워드
Spermatogenesis; Cell proliferation; Apoptosis;
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