백서 고환염전 복구 시 대측고환 손상 기전 및 Allopurinol 투여 효과
The Mechanism of Damage to the Contralateral Testis Following Testicular Torsion and Detorsion in Rats and the Effect of Allopurinol Administration
임대정, 홍성규, 정성진, 최황,
소속 상세정보
임대정 ( Lim Dae-Jung )
서울시립보라매병원 비뇨기과
홍성규 ( Hong Sung-Kyu )
서울대학교 의과대학 비뇨기과학교실
정성진 ( Jeong Seong-Jin )
서울대학교 의과대학 비뇨기과학교실
최황 ( Choi Hwang )
서울대학교 의과대학 비뇨기과학교실
KMID : 0358320060470020180
Abstract
Purpose: This study was performed to determine the mechanism of contralateral testis damage after repair of testicular torsion. In addition, we investigated the effect of allopurinol pretreatment on the contralateral testis damage.
Materials and Methods: The levels of malondialdehyde(MDA) in the testes, kidneys and serum samples were determined in rats following torsion, detorsion or detorsion after allopurinol pretreatment, as were the levels of superoxide dismutase(SOD) in the testes samples. H&E staining was performed on the testes specimens and we calculated the mean numbers of spermatids per tubule in each group.
Results: Torsion caused a significant increase in the MDA levels in the contralateral testes, and detorsion caused a further significant increase in MDA in the contralateral testes and in the serum. Moreover, pretreatment with allopurinol prevented this further increase. The levels of SOD in the contralateral testes were not significantly different between the sham and torsion groups, whereas detorsion caused a significant reduction in SOD. In addition, pretreatment with allopurinol also prevented this decrease. The contralateral testes were histologically normal in all groups and the mean numbers of spermatids per tubule in the ipsilateral and contralateral testes were similar in all groups.
Conclusion: Injury of the contralateral testis after the repair of testicular torsion seems to be caused by lipid peroxidation that is due to oxygen free radicals induced by the reperfusion of ischemic torsed testis. Toxic substances such as oxygen free radicals seem to be transferred to the contralateral testes through the blood stream, and allopurinol appears to protect the contralateral testes from the injury caused by detorsion. (Korean J Urol 2006;47:180-188)
키워드
Testis;Torsion;Reperfusion injury;Lipid peroxidation
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