백서 폐색 요관에서 세포고사 관련 유전자의 단백질 발현
The Protein Expressions of Apoptosis-associated Genes in the Obstructed Ureters of Rats
김형곤, 백성현, 노용수, 김현회, 곽철,
소속 상세정보
김형곤 ( Kim Hyeong-Gon )
건국대학교 의과대학 비뇨기과학교실
백성현 ( Paick Sung-Hyun )
건국대학교 의과대학 비뇨기과학교실
노용수 ( Lho Yong-Soo )
건국대학교 의과대학 비뇨기과학교실
김현회 ( Kim Hyeon-Hoe )
서울대학교 의과대학 비뇨기과학교실
곽철 ( Kwak Cheol )
서울대학교 의과대학 비뇨기과학교실
KMID : 0358320060470020189
Abstract
Purpose: The role of apoptosis in the pathogenesis of ureteral damage from obstructive uropathy has rarely been studied. This study was performed to determine the protein expression of the apoptosis-associated genes in the pathogenesis of ureteral damage during the course of obstructive uropathy in ligated rat ureters.
Materials and Methods: After unilateral ligation of the ureter, each group of five Sprague-Dawley rats was sacrificed and examined at 1, 5, 10, 15, 20, 25, 30 and 35 days after ligation: five rats with normal ureters were also examined as controls. The protein expressions of the fas-associated death domain(FADD), Bax, Bcl-xL and cyclooxygenases(COX)-2 genes in obstructed ureters were assessed by performing Western blotting.
Results: The expressions of FADD protein in the 20 and 25 day-obstructed ureters(DOUs) were significantly higher than that in control ureters and the peak was reached in the 25 DOUs. The expressions of Bcl-xL protein in the 20, 25 and 30 DOUs were significantly higher than that in the control ureters and the peak was reached in the 25 DOUs. The expression of COX-2 protein in the 5, 10, 15, 25 DOUs were significantly higher than that in the control ureters and the peak was reached in the 10 DOUs.
Conclusion: The FADD and Bcl-xL genes were involved in apoptosis of the obstructed ureter. The peaks of their expressions were at 25 DOUs. The expression of the COX-2 gene may be related with apoptosis in the obstructed ureter. (Korean J Urol 2006;47:189-194)
키워드
Ureteral obstruction;Apoptosis;Rats
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