흰쥐에서 Escherichia coli로 유도된 부고환염에 대한 Cobalt-Protoporphyrin의 항염증 효과
The Anti-inflammatory Effect of Cobalt-Protoporphyrin for Rats with Epididymitis Induced by Escherichia coli Infection
서일영, 정찬상, 임정식,
소속 상세정보
서일영 ( Seo Ill-Young )
원광대학교 의과대학 비뇨기과학교실
정찬상 ( Jeong Chan-Sang )
원광대학교 의과대학 비뇨기과학교실
임정식 ( Rim Joung-Sik )
원광대학교 의과대학 원광대학교병원 비뇨기과
KMID : 0358320060470060656
Abstract
Purpose: Heme oxygenase-1(HO-1), an inducible heat shock protein, catalyzes the heme to iron, biliverdin and carbon monoxide. It also has an inhibitory effect on necrosis and inflammation. Cobalt(III)-protoporphyrin IX(CoPP) is known to be a HO-1 inducer. Our intension was to find whether CoPP has an anti-inflammatory effect through the induction of HO-1 in rats with epididymitis.
Materials and Methods: Thirty two Sprague-Dawley male rats(age: 8-12 weeks, weight: 200-250gm) were selected for the experiments. Anesthesia was performed with an intraperitoneal injection of ketamine hydrochloride(140mg/kg). Four rats were taken and used as a control group. Epididymitis was induced in 28 rats by an injection of E. coli(1x105/ml) to the epididymis. In the first step, groups of 4 rats were sacrificed serially after 4, 12, 48, and 72 hours for Hematoxylin & Eosin(H&E) staining and Western blot for inducible nitric oxide synthase(iNOS) and cyclooxygenase(COX)-2. In the second step, groups of 4 rats were injected with either dimethyl sulphoxide(DMSO) 7μl, DMSO 7μl with 50mg/ml CoPP or DMSO 7μl with 100mg/ml CoPP. They were then sacrificed 72 hours later for H&E staining and Western blot for iNOS and COX-2.
Results: In the first step, increased inflammation was evident from the H&E staining over time. From the Western blots, iNOS expression was detected after 48 hours and COX-2 was after 12 hours. In the second step, decreased inflammation was evident from the H&E staining, and the expressions of iNOS and COX-2 were suppressed in the CoPP treated group.
Conclusions: CoPP can reduce the inflammation of epididymis in rats, and the mechanism may be related with HO-1. (Korean J Urol 2006;47: 656-660)
키워드
Cobalt protoporphyrin;Epididymitis;Heme oxygenase
원문 및 링크아웃 정보
등재저널 정보