트리올레인 지방 유상액을 이용한 혈고환장벽의 일시적 개방
Temporary Opening of the Testis-blood Barrier by Triolein Fat Emulsion
이상돈, 이완, 김학진,
소속 상세정보
이상돈 ( Lee Sang-Don )
부산대학교 의과대학 비뇨기과학교실
이완 ( Lee Wan )
부산대학교 의과대학 비뇨기과학교실
김학진 ( Kim Hak-Jin )
부산대학교 의과대학 영상의학교실
KMID : 0358320070480010087
Abstract
Purpose: Although the purpose of the blood-testis barrier(BTB) is to protect germ cells from harmful influences, it also impedes the delivery of chemotherapeutic agents to the testis. This study was undertaken to determine whether a triolein emulsion could transiently alter the permeability of the BTB in cats.
Materials & Methods: An emulsion of 0.05ml triolein in 20ml of saline or just 20ml of normal saline, as the control, were infused into the testicular arteries in 18 and 15 cats, respectively(embolic and control group). Pre- and post-contrast magnetic resonance images(MRIs) were obtained 30 minutes and 2 hours after embolization. Qualitative and quantitative analyses of the MRIs were performed via the presence and degree of contrast enhancement and the contrast enhancement ratios(CERs), respectively. An electron microscopy(EM) study was subsequently performed, using a lanthanum tracer, to correlate with the MRI results.
Results: Contrast enhancement of the testis was observed in both groups and at both time points, but was more prominent in the embolic group. The CERs in the embolic group were significantly higher than those in the control group(p=0.0001). In each group, the CERs at 2 hours were significantly lower than those at 30 minutes(p=0.006). In the EM study, the entry of lanthanum was markedly increased at 30 mins, but recovered at 2 hours after embolization compared to the control.
Conclusion: Intra-arterial infusion of triolein emulsion transiently increased the permeability of the BTB. This result may be useful in future studies for a chemotherapy delivery system to the testis. (Korean J Urol 2007;48:87-93)
키워드
Testis blood barrier;Fats;Emulsions;Cats
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