정상방광상피의 성선자극호르몬 분비호르몬(GnRH) 및 GnRH 수용체의 발현과 방광상피세포의 증식에 대한 GnRH의 역할
Gonadotropin Releasing Hormone(GnRH) and GnRH Receptor in Normal Bladder Epithelia and Their Role in Bladder Epithelial Proliferation
김명옥, 박문석, 이혜령, 이정희, 박종윤,
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김명옥 ( Kim Myoung-Ock )
경상대학교 자연과학대학 생물학교실
박문석 ( Park Moon-Seok )
경상대학교 자연과학대학 생물학교실
이혜령 ( Lee Hye-Lyoung )
경상대학교 자연과학대학 생물학교실
이정희 ( Lee Jeong-Hee )
경상대학교 의과대학 병리학교실
박종윤 ( Bahk Jong-Yoon )
경상대학교 의과대학 비뇨기과학교실
KMID : 0358320070480020152
Abstract
Purpose: To confirm the production of extra-hypothalamic gonadotropin releasing hormone(GnRH) and GnRH receptor in bladder mucosal epithelia, and a potential role of GnRH on the bladder, normal human bladder tissues, and primary cultured dog bladder mucosal epithelia were studied.
Materials & Methods: For this study, normal human bladder tissue from 4 patients and primary cultured normal bladder mucosal epithelial cells from 2 dogs were used. For localization of extra-hypothalamic GnRH and the extra-pituitary GnRH receptor, in situ hybridization and immunohistochemical staining were done. To evaluate the roles of exogenous GnRH in bladder mucosal cells, the culture media were supplemented with charcoal stripped serum and 4 different concentrations of GnRH(0, 10-3, 10-5 and 10-7M). The effect of exogenous GnRH was evaluated using a hemocytometer and fluorescence activated cell sorter(FACS).
Results: GnRH and GnRH receptors, and their mRNA signals were localized in most of the both human bladder mucosal epithelia and dog bladder mucosal epithelia, but not in a few cells. There were no significant GnRH effects on cellular proliferation and cell cycle changes(p＜0.05).
Conclusion: Bladder mucosal epithelium produces GnRH and GnRH receptors, but they do not effect either the proliferation or cell cycle changes. Although the exact function of extra-hypothalamic bladder GnRH is unknown, GnRH and GnRH receptors would be assumed to have unknown autocrine or paracrine relationships with each other. (Korean J Urol 2007;48:152-157)
키워드
Gonadotropin-releasing hormone;Receptor;Mucosa
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