만성전립선염에서 다중 중합효소연쇄반응을 이용한 잠복 미생물의 검출
Detection of Cryptic Microorganisms in Patients with Chronic Prostatitis by Multiplex Polymerase Chain Reaction
김태형, 김태흥, 명순철, 김영선, 김혜련, 이미경,
소속 상세정보
김태형 ( Kim Tae-Hyoung )
중앙대학교 의과대학 비뇨기과학교실
김태흥 ( Kim Tae-Heung )
중앙대학교 의과대학 비뇨기과학교실
명순철 ( Myung Soon-Chul )
중앙대학교 의과대학 비뇨기과학교실
김영선 ( Kim Young-Sun )
중앙대학교 의과대학 비뇨기과학교실
김혜련 ( Kim Hye-Ryun )
중앙대학교 의과대학 진단검사의학교실
이미경 ( Lee Mi-Kyung )
중앙대학교 의과대학 진단검사의학교실
KMID : 0358320070480030304
Abstract
Purpose: Chronic prostatitis frequently occurs in men of all ages. Recent studies suggest that fastidious microorganisms may play a role in chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS). The aim of this study was to evaluate the usefulness and significance of multiplex polymerase chain reaction(PCR) in the diagnosis of CP/CPPS.
Materials & Methods: First voided urine(FVU) and/or expressed prostatic secretions(EPS) were collected from 92 patients. Multiplex PCR, using Dual Specificity Oligo(DSOTM) primers, was used to test for Chlamydia trachomatis(CT), Mycoplasma genitalium(MG), Mycoplasma hominis(MH), Neisseria gonorrhoeae(NG), Trichomonas vaginalis(TV) and Ureaplasma urealyticum(UU).
Results: Multiplex PCR can be easily analyzed via visual comparison. Nine (39.1%) of the 23 CP/CPPS IIIa and 12(17.4%) of the 69 IIIb patients had positive multiplex PCR, with a total of 27 microorganisms isolated, including CT, MH, MG, UU, TV and NG in 9, 7, 4, 4, 2 and 1 case, respectively. Co-infections with 2 or 3 organisms occurred in 5 cases. For the samples collected from 32 patients for both FVU and EPS, 68.7% gave the same results.
Conclusion: Multiplex PCR, using DSOTM primers, can be useful for the simple detection of fastidious microorganisms in CP/CPPS. To achieve reliable results with multiplex PCR, feasible guidelines and standardization are of major importance. Further studies will be required to define the usefulness of molecular tests for CP/CPPS in clinical practice. (Korean J Urol 2007;48:304-309)
키워드
Prostatitis;Polymerase chain reaction
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