자가 근육줄기세포가 유착된 Hydroxyapatite/chitosan 복합체를 이용한 방광확대술
Augmentation Cystoplasty using Hydroxapatite/chitosan Composite Sheet Seeded with Autologous Muscle-derived Stem Cells
변석수, 정용식, 이상섭, 이햇님, 이지영, 이지열,
소속 상세정보
변석수 ( Byun Seok-Soo )
서울대학교 의과대학 비뇨기과학교실
정용식 ( Chung Yong-Sik )
전북대학교 섬유소재시스템공학과
이상섭 ( Lee Sang-Seob )
경기대학교 생물학과
이햇님 ( Lee Haet-Nim )
가톨릭대학교 의과대학 비뇨기과학교실
이지영 ( Lee Ji-Young )
가톨릭대학교 의과대학 비뇨기과학교실
이지열 ( Lee Ji-Youl )
가톨릭대학교 의과대학 비뇨기과학교실
KMID : 0358320070480040433
Abstract
Purpose: This study was designed to investigate the feasibility of a hydroxyapatite/chitosan(HAp/chitosan) composite, seeded with autologous muscle-derived stem cells, as a partial bladder substitute in rats.
Materials & Methods: Muscle-derived stem cells were isolated from the gastrocnemius muscle of 6 female Sprague-Dawley rats, using the preplate technique, and cultured on HAp/chitosan composite sheets. Sheets with 10mm diameters were implanted into the urinary bladder of rats following a hemicystectomy in an autologous fashion. Three rats were sacrificed 4 and 8 weeks postoperatively, and the morphological changes subsequently assessed by H&E and immunofluorescence staining using DAPI, myogenin and α-smooth muscle actin (SMA).
Results: All rats survived the scheduled duration. Adequate epithelialization was observed to be completed after postoperative week 4. Abundant muscle bundles, showing positive α-SMA staining, were observed after the 4th week. The bladder shape was well preserved after the 8th week. Ingrowing smooth muscles were observed on the periphery of the composite and muscular bundles, with positive myogenin immunostaining in the middle of the composite.
Conclusion: A HAp/chitosan composite sheet, seeded with autologous muscle-derived stem cells, showed a degree of skeletal muscle differentiation 8 weeks after augmentation cystoplasty, in an autologous fashion. This new material seeded with muscle-derived stem cells may, in the future, prove to be a viable option as a partial bladder substitute. (Korean J Urol 2007;48:433-438)
키워드
Bladder;Atrophy;Regeneration;Stem cells
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