흰쥐에서 요실금의 주사요법제로서 근육유래 줄기세포/키토산/수산화아파타이트 복합겔의 효과
Effects of Injection Therapy using Muscle Derived Stem Cell/Chitosan/Hydroapatite Composite Gel in a Rat Model of Urinary Incontinence
추관엽, 이지열, 박원희, 정용식,
소속 상세정보
추관엽 ( Choo Gwoan-Youb )
인하대학교 의과대학 비뇨기과학교실
이지열 ( Lee Ji-Youl )
가톨릭대학교 의과대학 비뇨기과학교실
박원희 ( Park Won-Hee )
인하대학교 의과대학 비뇨기과학교실
정용식 ( Jung Yong-Sik )
전북대학교 의과대학 비뇨기과학교실
KMID : 0358320070480060627
Abstract
Purpose: We investigated whether periurethral injections of muscle- derived stem cells(MDSC) and chitosan/hydroapatite after denervation of rat’s pudendal nerve could increase the leak point pressure over a long time period in a rat model of urinary incontinence.
Materials and Methods: Muscle-derived stem cells isolated from the gastrocnemius muscle of normal female rats were purified to obtain a myogenic population by using the preplate technique. The N group was the normal female rats, the D Group was the pudendal nerve transected group and the M Group was the MDSC/chitosan/hydroapatite composite gel injected group after pudendal nerve transection. The MDSC/chitosan/hydroapatite composite gel was injected into the proximal periurethral area. At 2 and 4 weeks, visually identified leak point pressure measurement was done with using the vertical tilt/intravesical pressure clamp model of urinary incontinence. The rats were then sacrificed and the periurethral tissues harvested for histological examination.
Results: The leak point pressure was significantly lower in the D group at each time compared with the N group, and the leak point pressure in the N and M groups were significantly higher than those in the D group at both 2 and 4 weeks. The persistence of MDSC over the period of study was verified by histological examination.
Conclusions: MDSC/chitosan/hydroapatite injection into the denervated external urethral sphincter in female rats increased the leak point pressure at 2 and 4 weeks. This MDSC/chitosan/hydroapatite composite gel can be an alternative injection method for treating urinary incontinence in the future.(Korean J Urol 2007;48:627-632)
키워드
Muscle;Stem cells;Urinary incontinence;Rats
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