편측성 완전 요관폐색 생쥐모델에서 NF-kappa B Decoy Oligodeoxynucleotide 투여 효과
The Effect of Ring-type NF-kappa B(NF-kB) Decoy Oligodeoxynucleotide on the Kidney for an Experimental Unilateral Ureteral Obstruction in Mice
권영근, 이영국, 강일, 이은석, 박관규, 금윤섭, 김경현, 박재신,
소속 상세정보
권영근 ( Kwon Young-Gun )
대구가톨릭대학교 의과대학 비뇨기과학교실
이영국 ( Lee Young-Guk )
대구가톨릭대학교 의과대학 비뇨기과학교실
강일 ( Kang Il )
대구가톨릭대학교 의과대학 비뇨기과학교실
이은석 ( Lee Eun-Seock )
대구가톨릭대학교 의과대학 비뇨기과학교실
박관규 ( Park Kwan-Kyu )
대구가톨릭대학교 의과대학 병리학교실
금윤섭 ( Kum Yoon-Seup )
대구가톨릭대학교 의과대학 병리학교실
김경현 ( Kim Kyung-Hyun )
대구가톨릭대학교 의과대학 병리학교실
박재신 ( Park Jae-Shin )
대구가톨릭대학교 의과대학 비뇨기과학교실
KMID : 0358320070480080815
Abstract
Purpose: The transcription factor, NF-kB, is important in the coordinated expressions of various pro-inflammatory and adhesion molecules. Our hypothesis is that inhibiting the action of NF-kB using a synthetic decoy oligodeoxynucleotide(ODN) can block the underlying inflammatory response in glomerulonephritis.
Materials and Methods: Forty two male C57BL6 mice, weighting 25g, were divided into four groups; in group 1, 2 mice were used as normal controls; in group 2, a unilateral ureteral obstruction(UUO) was induced in 12 mice; in group 3, 20 UUO mice were treated using ring-type NK-kB decoy ODN; and in group 4, 8 UUO mice were treated using scramble type NF-kB decoy ODN. The mice were killed 1, 3, 5 and 7 days after the NF-kB decoy ODN injections, and the blood urea nitrogen(BUN), and expressions of tumor necrosis factor-?(TNF-?), interleukin-?(IL-?), fibronectin, vascular cell adhesion molecule(VCAM) and monocyte chemotactic protein-1(MCP-1), as well as the histopathological findings, analyzed in each group.
Results: The serum levels of BUN, TNF-?, IL-?, fibronectin, VCAM and MCP-1 were increased in group 2, but decreased in group 3. The histopathological findings of the kidneys in group 3 were most similar to those found in the control(group 1).
Conclusions: NF-kB decoy ODN treatment substantially inhibited the disease, with reductions in the histological damage and the renal expressions of inflammatory cytokines.
키워드
NF-kappa B; Transcription factors; Ureteral obstruction
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