방광출구폐색에 동반된 배뇨근과활동성에서 Rho Kinase 억제제의 영향
Effects of Rho Kinase Inhibitor on Detrusor Overactivity after Bladder Outlet Obstruction in Rats
김현우, 조수연, 박덕진, 윤병일, 이지열,
소속 상세정보
김현우 ( Kim Hyun-Woo )
가톨릭대학교 의과대학 비뇨기과학교실
조수연 ( Cho Soo-Yeun )
가톨릭대학교 의과대학 비뇨기과학교실
박덕진 ( Park Duk-Jin )
가톨릭대학교 의과대학 비뇨기과학교실
윤병일 ( Yoon Byung-Il )
가톨릭대학교 의과대학 비뇨기과학교실
이지열 ( Lee Ji-Youl )
가톨릭대학교 의과대학 비뇨기과학교실
KMID : 0358320070480080832
Abstract
Purpose: A partial bladder outlet obstruction(PBOO) related detrusor hypertrophy is associated with up-regulation of the Rho kinase activity in an experimental animal model, and has been implicated in PBOO induced bladder dysfunction. The effect of a Rho kinase inhibitor on the voiding function in anesthetized rats with PBOO was investigated.
Materials and Methods: Eighteen Sprague-Dawley rats were divided into control(9 rats) and experimental(9 rats) groups. The experimental group was partially obstructed for 6 weeks, with cystometrograms(CMG) then were performed. The number of voids, and the intercontraction interval (ICI) and peak pressure(PP) were recorded. Rho kinase inhibitors were administered to the experimental group. An additional CMG was performed to observe the effects of Rho kinase inhibition. Bladder tissues were immunohistochemically(IHC) evaluated for the expression of RhoA protein.
Results: The bladder weights of the PBOO group were significantly increased compared with the control group(p＜0.05). Significant increases in the voiding frequency and PP, but a significant decrease in the ICI was observed in the PBOO group compared to the control group on the CMG (p＜0.05). The voiding frequency of the PBOO group was significantly decreased after Rho kinase inhibitor treatment(p＜0.05). The Rho kinase inhibitor treated group showed a decrease in the PP and an increase in the ICI compared to the PBOO group. The IHC showed a higher RhoA protein expression in the bladder tissues of the PBOO group.
Conclusions: H-1152, a specific inhibitor of Rho kinase, attenuates the PBOO-related detrusor overactivity in a rat model. The Rho kinase inhibitor appears to be a novel strategy for the management of bladder overactivity.
키워드
Urinary bladder neck obstruction; Rho-associated kinase; Rats
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