수신증을 유발한 백서 신장에서 p53, γ-H2AX, Ku70/Ku80의 발현
The Expressions of p53, ?-H2AX and Ku70/Ku80 That was Caused by Hydronephrosis in the Kidney of Rats
문찬, 문형윤, 김철성,
소속 상세정보
문찬 ( Moon Chan )
조선대학교 의과대학 비뇨기과학교실
문형윤 ( Moon Hyung-Yoon )
조선대학교 의과대학 비뇨기과학교실
김철성 ( Kim Chul-Sung )
조선대학교 의과대학 비뇨기과학교실
KMID : 0358320070480090976
Abstract
Purpose:There are little research about the factors of DNA damage and repair that might cause renal parenchymal damage under the condition of hydronephrosis. In this study, we studied the expressions of p53, ?-H2AX and Ku70/Ku80 in rat kidney under the condition of hydronephrosis.
Materials and Methods:16 Sprague-Dawley rats that were 7∼8 weeks old were used. Partial ureteral obstruction was induced in 12 rats. And for the other 4 rats, a sham-operation was done as a control. The hydronephrosis-induced rats had their right kidney removed after 1, 2 and 3 weeks and the sham-operated rats underwent nephrectomy after 3 weeks. Those removed tissues were examined with immunohistochemical staining and western blot to confirm the degree of expression of p53, ?-H2AX and Ku70/Ku80.
Results:The expressions of p53, ?-H2AX and Ku70/Ku80 caused by hydronephrosis in the rat increased as time passed, and these expressions of controls were in a low level or they were negative. These results were similar with the results of the immunohistochemical staining and Western blot.
Conclusions:This study confirmed that the protein expressions of ?- H2AX, p53, and Ku70/Ku80 are increased, and these expressions are the DNA damage-related factors in the renal parenchyma of hydronephrosis- induced rats. We confirmed the possibility that DNA double strand breaks (DSBs) might be the main mechanism that induces renal parenchymal damage under the condition of hydronephrosis. (Korean J Urol 2007;48: 976-983)
키워드
Hydronephrosis;DNA damage;Double-strand DNA breaks;DNA repair
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