요실금 모델 쥐에서 근육줄기세포/Alginate/Polycaprolactone 복합체의 주사요법
Muscle Derived Stem Cell/Alginate/Polycaprolactone/ Injection Therapy in Rats with Denervated Urethral Sphincter
이햇님, 김현우, 변석수, 이상섭, 이지영, 이지열, 고준성,
소속 상세정보
이햇님 ( Lee Haet-Nim )
가톨릭대학교 의과대학 비뇨기과학교실
김현우 ( Kim Hyun-Woo )
가톨릭대학교 의과대학 비뇨기과학교실
변석수 ( Byun Seok-Soo )
서울대학교 의과대학 비뇨기과학교실
이상섭 ( Lee Sang-Seob )
경기대학교 생물학과
이지영 ( Lee Ji-Young )
가톨릭대학교 의과대학 비뇨기과학교실
이지열 ( Lee Ji-Youl )
가톨릭대학교 의과대학 비뇨기과학교실
고준성 ( Koh Jun-Sung )
가톨릭대학교 의과대학 비뇨기과학교실
KMID : 0358320070480121296
Abstract
Purpose : In this study, we tested whether injections of muscle-derived stem cells and alginate(Alg)/polycaprolactone(PCL) after denervation of the pudendal nerve could increase the leak point pressure(LPP) and closing pressure(CP) over the long term in a rat model of urinary incontinence.
Material and Methods : Muscle derived stem cells(MDSC) were isolated from the gastrocnemius muscle of normal female rats, and these cells were purified for creating a myogenic population by the preplate technique. In the denervated(D) group, the pudendal nerve was transected bilaterally via a dorsal incision in order to denervate the external urethral sphincter. The denervated external urethral sphincter was injected with Alg/PCL(AP group), or MDSC/Alg/PCL(M group) into the proximal urethra after pudendal nerve transection. At 1 and 3 months, the LPP and CP measurements were visually identified by using the vertical tilt/intravesical pressure clamp model of stress urinary incontinence. The rats were then sacrificed and their urethras were harvested for histology.
Results : Both the LPP and CP were significantly lower in the denervated group at each time compared with the normal(N group), AP and M groups, and both the LPP and CP in the N, AP and M groups were significantly higher than those in the D group at both 1 and 3 months. The persistence of MDSC over the period of the study was verified by histology. Thus, pudendal nerve denervation led to a progressive decline in the LPP and CP that was evident at 1 month and this persisted to 3 months, and injection of MDSC/Alg/PCL into the denervated rats led to a long term increase in the LPP and CP.
Conclusion : The N, AP and M groups all had significantly higher LPPs than the D group, and MDSC/Alg/PCL injection into the denervated external urethral sphincter in female rats increased the LPP and CP in both the short and long term. We also observed a long term bulking effect of MDSC/Alg/PCL injection in the stress incontinence animal model. (Korean J Urol 2007;48:1296-1301)
키워드
Muscle derived stem cell;Alginate;Polycaprolactone;Urinary incontinence
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