수컷 백서의 전기자극 인공사정
Electroejaculation in the Male Rat
백성현, 이병기, 윤성욱, 백민기, 김형곤, 송은영, 류영수, 노용수,
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백성현 ( Paick Sung-Hyun )
건국대학교 의과대학 비뇨기과학교실
이병기 ( Lee Byung-Ki )
건국대학교 의과대학 비뇨기과학교실
윤성욱 ( Yoon Sung-Wook )
건국대학교 의과대학 비뇨기과학교실
백민기 ( Baek Min-Ki )
건국대학교 의과대학 비뇨기과학교실
김형곤 ( Kim Hyeong-Gon )
건국대학교 의과대학 비뇨기과학교실
송은영 ( Song Eun-Young )
건국대학교 의과대학 임상병리학교실
류영수 ( Lyoo Young-Soo )
건국대학교 수의과대학 수의병리학교실
노용수 ( Lho Yong-Soo )
건국대학교 의과대학 비뇨기과학교실
KMID : 0358320080490030266
Abstract
Purpose: We examined the possibility of electroejaculation for the evaluation of fertility in the male rat.
Materials and Methods: Ten 8-week-old Sprague-Dawley male rats that underwent seminal vesiculectomy were used for semen collection by electroejaculation. With a transrectal probe(60Hz, 3V, 0.5A), sign wave electric stimulation was applied to an anesthetized rat. Ejaculated semen was collected by a pipette and the volume and sperm density of the ejaculate were analyzed. Two weeks later, a second electroejaculation was performed under the same conditions with the same rats.
Results: At the first attempt, all 10 rats ejaculated following electric stimulation. The mean semen volume was 8.9?l(range, 3.0-28.5?l) and the mean sperm density was 6,428/ml(range, 320-20,997/ml). At the second attempt, only 7 of 10 rats(70%) ejaculated. The mean semen volume was 5.6?l(range, 3.3-8.6?l) and the mean sperm density was 2,801/ml (range, 227-12,555/ml).
Conclusions: These results indicated that electroejaculation has the possibility of being a useful method for evaluation of fertility in the male rat. (Korean J Urol 2008;49:268-272)
키워드
Electric stimulation;Ejaculation;Fertility;Rats
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