방광암세포주의 항암제 반응에 대한 in vivo Hollow Fiber Assay
In vivo Hollow Fiber Assay for Anticancer Drugs’ Responsiveness in a Bladder Cancer Model
문기혁, 정성진, 홍성규, 변석수, 이상은, 한병규,
소속 상세정보
문기혁 ( Moon Ki-Hyuck )
서울대학교 의과대학 비뇨기과학교실
정성진 ( Jeong Seong-Jin )
서울대학교 의과대학 비뇨기과학교실
홍성규 ( Hong Sung-Kyu )
서울대학교 의과대학 비뇨기과학교실
변석수 ( Byun Seok-Soo )
서울대학교 의과대학 비뇨기과학교실
이상은 ( Lee Sang-Eun )
서울대학교 의과대학 비뇨기과학교실
한병규 ( Han Byoung-Kyu )
서울대학교 의과대학 비뇨기과학교실
KMID : 0358320080490050392
Abstract
Purpose: The National Cancer Institute(NCI)’s Hollow Fiber Assay(HFA) is currently used as an in vivo screening model to quantitatively define anticancer activity. To investigate the use of HFA in a bladder cancer model, we conducted in vitro and in vivo experiments with several anticancer drugs in nude mice.
Materials and Methods: The human bladder cancer cell lines(CRL2742, 253JP, SW1710, HTB9) were cultured both in vitro and in vivo in polyvinylidene fluoride(PVDF) hollow fibers. The fibers were implanted intraperitoneally(ip) and subcutaneously(sc) into female athymic nude mice(C57BL/6), and the mice were then treated with gemcitabine 120 mg/kg(bolus), cisplatin(3mg/kg), paclitaxel(15mg/kg) or vehicle only (control) for 4-consecutive days. After 6 days, the fibers were retrieved and the viable cell density was analyzed by MTT assay.
Results: The difference between in vitro and in vivo growth was not significant for the CRL2742, 253J-P and SW1710 cell lines; the difference between the ip and sc fibers was also not significant in the CRL2742, SW1710 and HTB9 cell lines. After drug treatment, the percent of growth inhibition revealed constant and effective anticancer activities for the 3 individual drugs.
Conclusions: This study demonstrates the possibility of measuring and quantifying the anticancer effect with using in vivo hollow fiber assay in a bladder cancer model.
키워드
Hollow fiber assay;Bladder;Neoplasm
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