난소 절제한 백서의 방광과 요도에서 Neuronal 및 Endothelial Nitric Oxide Synthase의 발현과 조직학적 변화
The Effect of Estrogen on Expression of Neuronal and Endothelial Nitric Oxide Synthase and on Histologic Composition in Rat Bladder and Urethra
박성우, 이정주,
소속 상세정보
박성우 ( Park Sung-Woo )
부산대학교 의과대학 비뇨기과학교실
이정주 ( Lee Jeong-Zoo )
부산대학교 의과대학 비뇨기과학교실
KMID : 0358320080490060533
Abstract
Purpose: We investigated the effects of estrogen on the expression of neuronal nitric oxide synthase(nNOS) and on endothelial nitric oxide synthase(eNOS), and furthermore elucidated its effect on the histologic composition of the rat bladder and urethra.
Material and Methods :Forty-five mature female Sprague-Dawley rats (10-11 weeks, 235-250g) were randomly assigned to one of three groups: control group(C), oophorectomy group(O), or hormone(estradiol) replacement group(H). The degree of expression of nNOS and eNOS in bladder and urethral tissues was investigated using immunohistochemical staining, and tissue was described as mucosa, smooth muscle, or vessel. We also investigated changes in histologic composition using Masson’s trichrome stain.
Results:In bladder and urethra, the expression rates of nNOS and eNOS significantly increased in the O group, but decreased in the H group (p＜ 0.05), except for nNOS in the mucosa and smooth muscle of the bladder. The relative collagen ratios significantly increased in bladder and urethra in the O group, to 121.2±12.7% and 135.5±25.2%, respectively, but decreased to 95.6±15.2% and 93.7±12.8% in the H group, respectively (p＜0.05).
Conclusion:These data suggest that estrogen replacement therapy decreases nNOS and eNOS expression and the collagen component in the urethra.
키워드
Nitric oxide;synthase; Collagen; Incontinence
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