마이크로-CT를 이용한 흰쥐 신장의 미세혈관구조 관찰
Evaluation of Renal Microvasculature Using Micro-computed Tomography in Rat
유승현, 서일영, 윤권하,
소속 상세정보
유승현 ( You Seung-Hyun )
원광대학교 의과대학 비뇨기과학교실
서일영 ( Seo Ill-Young )
원광대학교 의과대학 비뇨기과학교실
윤권하 ( Yoon Kwon-Ha )
원광대학교 의과대학 영상의학교실
KMID : 0358320080490080669
Abstract
Purpose: Rodent models that mimic human renal diseases are being increasingly recognized as powerful tools in the development of new drugs and for evaluating the efficacy of novel therapeutics in a preclinical setting. However, there are few reports on microvasculature imaging of the urinary system in small animals. An experimental study was performed to evaluate the microvasculature in a rat kidney using micro- computed tomography(CT) with three-dimensional images. Materials and
Methods: Five Sprague-Dawley male rats(age: 10-12 weeks, weight: 200-250g) underwent a laparotomy under anesthesia with an intramuscular injection of 0.5cc xylazine hydrochloride and ketamine mixed solution(1:10). After ligation of the abdominal aorta and inferior vena cava immediately above the renal artery, a 24 gazed catheter was inserted into the abdominal aorta. A physiological solution and heparin(500U) were infused through the catheter to flush the blood from the renal vasculature. The kidney was enhanced using self-made contrast material. The excised kidney was frozen for the micro-CT scan.
Results: The mean longitudinal diameter and weight of the 10 resected kidneys was 1.95±0.15cm and 2.0±0.28g, respectively. The images were represented by three-dimensional arrays of cubic voxels with opacities in the blood vessels. In the section taken from the arrays, four regions of the kidney could be identified easily by their characteristic vascular features.
Conclusions: Micro-CT is a promising method for evaluating the renal microvascular architecture in a rat kidney. It can for the foundation of an experimental study aimed at providing quantitative information on the urinary system in a rodent model. (Korean J Urol 2008;49:669-674)
키워드
Kidney;Imaging;three-dimensional;Microcirculation;Vasculature
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