신세포암 동물모델에서 전신적 면역치료와 동반한 국소 냉동요법의 항암 효과
Antitumor Effect of in Situ Cryoablation with Systemic Immunotherapy on Murine Renal Cell Tumor
신동식, 배재현, 박홍석, 문두건, 강석호, 천준, 윤덕기, 고영휘, 최훈,
소속 상세정보
신동식 ( Shin Dong-Shik )
고려대학교 의과대학 비뇨기과학교실
배재현 ( Bae Jae-Hyun )
고려대학교 의과대학 비뇨기과학교실
박홍석 ( Park Hong-Seok )
고려대학교 의과대학 비뇨기과학교실
문두건 ( Moon Du-Geon )
고려대학교 의과대학 비뇨기과학교실
강석호 ( Kang Seok-Ho )
고려대학교 의과대학 비뇨기과학교실
천준 ( Cheon Jun )
고려대학교 의과대학 비뇨기과학교실
윤덕기 ( Yoon Duck-Ki )
고려대학교 의과대학 비뇨기과학교실
고영휘 ( Ko Young-Hwii )
고려대학교 의과대학 비뇨기과학교실
최훈 ( Choi Hoon )
고려대학교 의과대학 비뇨기과학교실
KMID : 0358320080490110965
Abstract
Purpose: To investigate synergistic effect of local cryoablation with systemic immunotherapy, the tumor control ability and immunologic responses of combining these two modalities was compared with that of cryoablation, surgical excision, and immunotherapy only group in a tumor re-challenge model.
Materials and Methods: Preliminary experiments were performed in two stages. The first stage consisted of 36 Balb/c mice with Renca bearing tumors imbedded in the right thigh, and was treated with interleukin-2 (IL-2) and interferon-alpha(IFN-?) to evaluate the efficacy of immunotherapy and to determine the adequate dosage. The second stage was performed on 10 mice, to evaluate histological changes and efficacy after cryoablation. The main experiment was performed on 48 mice, divided into 6 groups of control with tumor implantation, excision of tumor, excision combined with immunotherapy, cryoablation of tumor, cryoablation with immunotherapy and control without tumor. After treatment, tumor re-challenge was performed with Renca cell, then the growth pattern was evaluated with physical measurements, and immune response was investigated with fluorescent activated cell sorter and cytotoxicity assay.
Results: Preliminary studies on immunologic efficacy revealed that IL-2 and IFN-? have a dose dependent inhibition of tumor growth. The main experiment evaluating the efficacy of combination treatment revealed that cryoablation with immunotherapy proved to be most effective in terms of tumor recurrence and tumor growth inhibition, yet the difference was not statistically significant from monotherapy with cryoablation. However, cytotoxicity was significantly increased cryoablation with immunotherapy compared with other groups.
Conclusions: Cryoablation on tumor re-challenge mice model showed advantages with immunotherapy most prominently in cytotoxicity.
키워드
Cryoablation;Immunotherapy;Renal cell carcinoma
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