전립샘암 세포주에서 ?-Catenin 및 Matrix Metalloproteinase-7의 발현에 대한 Decursin의 효과
Effect of Decursin on the Expression of ?-Catenin and Matrix Metalloproteinase-7 in Prostate Cancer Cell Lines
최익준, 민권식, 강동일, 송규용, 오상택,
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최익준 ( Choi Ik-Joon )
인제대학교 의과대학 비뇨기과학교실
민권식 ( Min Kweon-Sik )
인제대학교 의과대학 비뇨기과학교실
강동일 ( Kang Dong-Il )
인제대학교 의과대학 비뇨기과학교실
송규용 ( Song Gyu-Yong )
충남대학교 약학대학
오상택 ( Oh Sang-Taek )
인제대학교 약물유전체연구센터
KMID : 0358320090500010081
Abstract
Purpose: Alterations in the Wnt/?-catenin pathway are associated with the development and progression of human prostate cancer. Decursin can attenuate the Wnt/?-catenin pathway. We investigated the relationship between the Wnt/?-catenin pathway and decursin in prostate cancer cells.
Materials and Methods: PC-3 and LNCaP cell lines were used. Cell viability was measured with methyl-thiazole tetrazolium bromide(MTT) assays, and cell apoptosis analysis was performed by FACScan. The amount of ?-catenin protein after treatment with decursin was measured by Western blot analysis. Expression of MMP-7 mRNA was detected by real-time polymerase chain reaction(RT-PCR).
Results: Death and apoptosis were increased after treatment with decursin 0.5-100?M in PC-3 and LNCaP cells. This was revealed dose and time-dependent increase of cancer cell death on 24, 48 and 72 hours. FACScan showed an increment of apoptosis on 24, 48 hours. Expression of intracellular ?-catenin protein was decreased dose-dependently in both of prostate cancer cell lines. Decursin reduced MMP-7 mRNA expression on 6, 12, 24, 48 hours dose-dependently.
Conclusions: Decursin affects the viability of prostate cancer cells. Increased cancer cell death was associated with increased apoptosis. This study suggests that decursin may play a role in the treatment of prostate cancer. (Korean J Urol 2009;50:81-88)
키워드
Decursin;Beta-catenin;Matrix metalloproteinase;Prostate
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