줄기세포와 조직공학적 기법을 이용한 회장도관술 대체에 대한 예비연구
Preliminary Study of Tissue-Engineered Ileal Conduit Using Poly (epsilon-Caprolactone) (PCL) Nano-Sheet Seeded with Muscle-Derived Stem Cells
이용석, 김현우, 조수연, 이지영, 이지열, 김희연, 강세희, 권은비, 홍성갑,
소속 상세정보
이용석 ( Lee Yong-Seok )
가톨릭대학교 의과대학 비뇨기과학교실
김현우 ( Kim Hyun-Woo )
가톨릭대학교 의과대학 비뇨기과학교실
조수연 ( Cho Soo-Yeun )
가톨릭대학교 의과대학 비뇨기과학교실
이지영 ( Lee Ji-Young )
가톨릭대학교 의과대학 비뇨기과학교실
이지열 ( Lee Ji-Youl )
가톨릭대학교 의과대학 비뇨기과학교실
김희연 ( Kim Hee-Yeon )
가톨릭대학교 의과대학 비뇨기과학교실
강세희 ( Kang Se-Hee )
가톨릭대학교 의과대학 비뇨기과학교실
권은비 ( Kwon Eun-Bi )
가톨릭대학교 의과대학 비뇨기과학교실
홍성갑 ( Hong Song-Karp )
가톨릭대학교 의과대학 비뇨기과학교실
KMID : 0358320090500030282
Abstract
Purpose: We studied the feasibility and results of a tissue-engineered ileal conduit using a poly (epsilon-caprolactone) (PCL) nano-sheet seeded with muscle-derived stem cells to replace a conventional ileal conduit in rats.
Materials and methos: Muscle-derived stem cells were isolated from the gastrocnemius muscle of female Sprague-Dawley rats (200-250 g, n=6) by use of a preplate technique and were cultured on a PCL nano-sheet. The PCL nano-sheet was implanted into the omentum of rats and was then made into a conical shaped conduit. Rats were sacrificed 4 and 8 weeks after implantation, and morphologic changes were assessed by H&E and immunofluorescence staining, including DAPI staining and staining for myogenin and myosin heavy chain (MyHC).
Results: All rats survived until the end of the experiment. A minimal inflammatory reaction was observed around the PCL nano-sheet in the 4 week specimens but was found to be reduced in the 8 week specimen. Muscle bundles were identified at week 4 as well as week 8 after implantation on H&E staining. Around the PCL sheet, immunostaining for both myogenin and MyHC were positive, indicating skeletal muscle differentiation and ingrowth into the PCL sheet.
Conclusions: A PCL nano-sheet seeded with muscle-derived stem cells showed successful skeletal muscle differentiation at 4 and 8 weeks after implantation. This preliminary result supports the feasibility of a tissue-engineered ileal conduit using a PCL nano-sheet (seeded with muscle-derived stem cells) in place of conventional ileal conduits.
키워드
Urinary diversion;Tissue engineering;Stem cells
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